Application of quantitative real-time polymerase chain reaction on the assessment of organophosphorus compound degradation in in situ soil

Yunyoung Kwak; Sang Jun Kim; In Koo Rhee; Jae Ho Shin

doi:10.1007/s13765-012-2168-4

Application of quantitative real-time polymerase chain reaction on the assessment of organophosphorus compound degradation in in situ soil

Yunyoung Kwak, Sang Jun Kim, In Koo Rhee, Jae Ho Shin

School of Applied Bioscience

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Quantitative real-time PCR (qPCR) method was applied to quantify the functional gene encoding organophosphorus hydrolase for assessing the degradation efficacy by bacterial strains on an organophosphorus compound in in situ soil. The specific primers targeting the organophosphorus hydrolase were designed and tested on reference bacterial strains and in DNA samples extracted from in situ soil samples contaminated by an organophosphorus compound. The established qPCR assay is a practical method for the analysis of in situ soil samples undergoing bioremediation of organophosphorus compounds.

Original language	English
Pages (from-to)	757-763
Number of pages	7
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume	55
Issue number	6
DOIs	https://doi.org/10.1007/s13765-012-2168-4
State	Published - Dec 2012

Keywords

biodegradation
opd gene
organophosphorus hydrolase
quantitative real-time polymerase chain reaction

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10.1007/s13765-012-2168-4

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abstract = "Quantitative real-time PCR (qPCR) method was applied to quantify the functional gene encoding organophosphorus hydrolase for assessing the degradation efficacy by bacterial strains on an organophosphorus compound in in situ soil. The specific primers targeting the organophosphorus hydrolase were designed and tested on reference bacterial strains and in DNA samples extracted from in situ soil samples contaminated by an organophosphorus compound. The established qPCR assay is a practical method for the analysis of in situ soil samples undergoing bioremediation of organophosphorus compounds.",

keywords = "biodegradation, opd gene, organophosphorus hydrolase, quantitative real-time polymerase chain reaction",

author = "Yunyoung Kwak and Kim, {Sang Jun} and Rhee, {In Koo} and Shin, {Jae Ho}",

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AU - Kwak, Yunyoung

AU - Kim, Sang Jun

AU - Rhee, In Koo

AU - Shin, Jae Ho

PY - 2012/12

Y1 - 2012/12

N2 - Quantitative real-time PCR (qPCR) method was applied to quantify the functional gene encoding organophosphorus hydrolase for assessing the degradation efficacy by bacterial strains on an organophosphorus compound in in situ soil. The specific primers targeting the organophosphorus hydrolase were designed and tested on reference bacterial strains and in DNA samples extracted from in situ soil samples contaminated by an organophosphorus compound. The established qPCR assay is a practical method for the analysis of in situ soil samples undergoing bioremediation of organophosphorus compounds.

AB - Quantitative real-time PCR (qPCR) method was applied to quantify the functional gene encoding organophosphorus hydrolase for assessing the degradation efficacy by bacterial strains on an organophosphorus compound in in situ soil. The specific primers targeting the organophosphorus hydrolase were designed and tested on reference bacterial strains and in DNA samples extracted from in situ soil samples contaminated by an organophosphorus compound. The established qPCR assay is a practical method for the analysis of in situ soil samples undergoing bioremediation of organophosphorus compounds.

KW - biodegradation

KW - opd gene

KW - organophosphorus hydrolase

KW - quantitative real-time polymerase chain reaction

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JO - Journal of the Korean Society for Applied Biological Chemistry

JF - Journal of the Korean Society for Applied Biological Chemistry

IS - 6

ER -

Application of quantitative real-time polymerase chain reaction on the assessment of organophosphorus compound degradation in in situ soil

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Keywords

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