Archaeal minichromosome maintenance (MCM) helicase can unwind DNA bound by archaeal histones and transcription factors

Jae Ho Shin, Thomas J. Santangelo, Yunwei Xie, John N. Reeve, Zvi Kelman

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Protein-DNA complexes must be disassembled to facilitate DNA replication. Replication forks contain a helicase that unwinds the duplex DNA at the front of the fork. The minichromosome maintenance helicase from the archaeon Methanothermobacter thermautotrophicus required only ATP to unwind DNA bound into complexes by the M. thermautotrophicus archaeal histone HMtA2, transcription repressor TrpY, or into a transcription pre-initiation complex by M. thermautotrophicus TATA-box-binding protein, transcription factor B, and RNA polymerase. In contrast, the minichromosome maintenance helicase was unable to unwind DNA bound by this archaeal RNA polymerase in a stalled transcript-elongating complex.

Original languageEnglish
Pages (from-to)4908-4915
Number of pages8
JournalJournal of Biological Chemistry
Volume282
Issue number7
DOIs
StatePublished - 16 Feb 2007

Fingerprint

Dive into the research topics of 'Archaeal minichromosome maintenance (MCM) helicase can unwind DNA bound by archaeal histones and transcription factors'. Together they form a unique fingerprint.

Cite this