Assessment of peanut allergen Ara h1 in processed foods using a SWCNTs-based nanobiosensor

Abdus Sobhan; Jun Hyun Oh; Mi Kyung Park; Seung Wook Kim; Chulhwan Park; Jinyoung Lee

doi:10.1080/09168451.2018.1453295

Assessment of peanut allergen Ara h1 in processed foods using a SWCNTs-based nanobiosensor

Abdus Sobhan, Jun Hyun Oh, Mi Kyung Park, Seung Wook Kim, Chulhwan Park, Jinyoung Lee

School of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The goals of this research were to develop a rapid single-walled carbon nanotube (SWCNT)-based biosensor and to employ it to commercial food products for Ara h1 detection. The SWCNT-based biosensor was fabricated with SWCNTs immobilized with antibody (pAb) through hybridization of 1-pyrenebutanoic acid succinimidyl ester (1-PBASE) as a linker. The resistance difference (ΔR) was calculated by measuring linear sweep voltammetry (LSV) using a potentiostat. Resistance values increased as the concentration of Ara h1 increased over the range of 1 to 10⁵ ng/L. The specific binding of anti-Ara h1 pAb to antigen including Ara h1 was confirmed by both indirect ELISA kit and biosensor assay. The biosensor was exposed to extracts prepared from commercial processed food containing peanuts, or no peanuts, and could successfully distinguish the peanut containing foods. In addition, the application of present biosensor approach documented the precise detection of Ara h1 concentrations in commercially available peanut containing foods.

Original language	English
Pages (from-to)	1134-1142
Number of pages	9
Journal	Bioscience, Biotechnology and Biochemistry
Volume	82
Issue number	7
DOIs	https://doi.org/10.1080/09168451.2018.1453295
State	Published - 2018

Keywords

Ara h1
Nanobiosensor
Peanut allergy
Peanut-process foods
Single walled carbon nanotube

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10.1080/09168451.2018.1453295

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title = "Assessment of peanut allergen Ara h1 in processed foods using a SWCNTs-based nanobiosensor",

abstract = "The goals of this research were to develop a rapid single-walled carbon nanotube (SWCNT)-based biosensor and to employ it to commercial food products for Ara h1 detection. The SWCNT-based biosensor was fabricated with SWCNTs immobilized with antibody (pAb) through hybridization of 1-pyrenebutanoic acid succinimidyl ester (1-PBASE) as a linker. The resistance difference (ΔR) was calculated by measuring linear sweep voltammetry (LSV) using a potentiostat. Resistance values increased as the concentration of Ara h1 increased over the range of 1 to 105 ng/L. The specific binding of anti-Ara h1 pAb to antigen including Ara h1 was confirmed by both indirect ELISA kit and biosensor assay. The biosensor was exposed to extracts prepared from commercial processed food containing peanuts, or no peanuts, and could successfully distinguish the peanut containing foods. In addition, the application of present biosensor approach documented the precise detection of Ara h1 concentrations in commercially available peanut containing foods.",

keywords = "Ara h1, Nanobiosensor, Peanut allergy, Peanut-process foods, Single walled carbon nanotube",

author = "Abdus Sobhan and Oh, {Jun Hyun} and Park, {Mi Kyung} and Kim, {Seung Wook} and Chulhwan Park and Jinyoung Lee",

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doi = "10.1080/09168451.2018.1453295",

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AU - Oh, Jun Hyun

AU - Park, Mi Kyung

AU - Kim, Seung Wook

AU - Park, Chulhwan

AU - Lee, Jinyoung

PY - 2018

Y1 - 2018

N2 - The goals of this research were to develop a rapid single-walled carbon nanotube (SWCNT)-based biosensor and to employ it to commercial food products for Ara h1 detection. The SWCNT-based biosensor was fabricated with SWCNTs immobilized with antibody (pAb) through hybridization of 1-pyrenebutanoic acid succinimidyl ester (1-PBASE) as a linker. The resistance difference (ΔR) was calculated by measuring linear sweep voltammetry (LSV) using a potentiostat. Resistance values increased as the concentration of Ara h1 increased over the range of 1 to 105 ng/L. The specific binding of anti-Ara h1 pAb to antigen including Ara h1 was confirmed by both indirect ELISA kit and biosensor assay. The biosensor was exposed to extracts prepared from commercial processed food containing peanuts, or no peanuts, and could successfully distinguish the peanut containing foods. In addition, the application of present biosensor approach documented the precise detection of Ara h1 concentrations in commercially available peanut containing foods.

AB - The goals of this research were to develop a rapid single-walled carbon nanotube (SWCNT)-based biosensor and to employ it to commercial food products for Ara h1 detection. The SWCNT-based biosensor was fabricated with SWCNTs immobilized with antibody (pAb) through hybridization of 1-pyrenebutanoic acid succinimidyl ester (1-PBASE) as a linker. The resistance difference (ΔR) was calculated by measuring linear sweep voltammetry (LSV) using a potentiostat. Resistance values increased as the concentration of Ara h1 increased over the range of 1 to 105 ng/L. The specific binding of anti-Ara h1 pAb to antigen including Ara h1 was confirmed by both indirect ELISA kit and biosensor assay. The biosensor was exposed to extracts prepared from commercial processed food containing peanuts, or no peanuts, and could successfully distinguish the peanut containing foods. In addition, the application of present biosensor approach documented the precise detection of Ara h1 concentrations in commercially available peanut containing foods.

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KW - Nanobiosensor

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KW - Peanut-process foods

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Assessment of peanut allergen Ara h1 in processed foods using a SWCNTs-based nanobiosensor

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