Assessment of the genetic diversity of rice germplasms characterized by black-purple and red pericarp color using simple sequence repeat markers

Jae Ryoung Park, Won Tae Yang, Yong Sham Kwon, Hyeon Nam Kim, Kyung Min Kim, Doh Hoon Kim

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The assessment of the genetic diversity within germplasm collections can be accomplished using simple sequence repeat (SSR) markers and association mapping techniques. The present study was conducted to evaluate the genetic diversity of a colored rice germplasm collection containing 376 black-purple rice samples and 172 red pericarp samples, conserved by Dong-A University. There were 600 pairs of SSR primers screened against 11 rice varieties. Sixteen informative primer pairs were selected, having high polymorphism information content (PIC) values, which were then used to assess the genetic diversity within the collection. A total of 409 polymorphic amplified fragments were obtained using the 16 SSR markers. The number of alleles per locus ranged from 11 to 47, with an average of 25.6. The average PIC value was 0.913, ranging from 0.855 to 0.964. Four hundred and nine SSR loci were used to calculate Jaccard’s distance coefficients, using the unweighted pair-group method with arithmetic mean cluster analysis. These accessions were separated into several distinctive groups corresponding to their morphology. The results provided valuable information for the colored rice breeding program and showed the importance of protecting germplasm resources and the molecular markers that can be derived from them.

Original languageEnglish
Article number471
JournalPlants
Volume8
Issue number11
DOIs
StatePublished - Nov 2019

Keywords

  • Cultivar identification
  • Molecular marker
  • Plant variety protection
  • Rice (Oryza sativa L.)
  • Simple sequence repeat (SSR)

Fingerprint

Dive into the research topics of 'Assessment of the genetic diversity of rice germplasms characterized by black-purple and red pericarp color using simple sequence repeat markers'. Together they form a unique fingerprint.

Cite this