Abstract
Secondary cell wall is the most abundant biomass produced by plants. Plant secondary cell wall is composed of a complex mixture of cellulose, hemicellulose, and lignin. Lignin, a phenolic polymer that hinders the degradation of cell wall polysaccharides to simple sugars destined for fermentation to bioethanol. Cell wall biosynthesis pathway-specific biomass engineering offers an attractive ‘genetic pretreatment’ strategy to improve bioenergy feedstock. Recently, we found a transcription factor, MYB7, which is a transcriptional switch that may turns off the genes necessary for lignin biosynthesis. To gain insights into MYB7 mediated transcriptional regulation, we first established a dominant suppression system in Arabidopsis by expressing MYB7-SRDX. Then we used a transient transcriptional activation assay to confirm that MYB7 suppress the transcription of the lignin biosynthetic gene. Taken together, we conclude that MYB7 function as a repressor of the genes involved in the lignin biosynthesis.
| Original language | English |
|---|---|
| Pages (from-to) | 215-220 |
| Number of pages | 6 |
| Journal | Journal of Applied Biological Chemistry |
| Volume | 59 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 2016 |
Keywords
- Bioethanol
- Lignin
- MYB7
- Secondary wall biosynthesis
- Transcriptional regulation
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