Automated Platform for Rapid and Reproducible Sample Preparations in Point-of-Care(POC) Molecular Diagnostics

Dongkyu Lee; Ohwon Kwon; Kang Ho Lee; Jongsu Yun; Changwon kim; Joonho Seo; Jeawan Koo; Gyu Man Kim

doi:10.1007/s13206-019-3304-6

Automated Platform for Rapid and Reproducible Sample Preparations in Point-of-Care(POC) Molecular Diagnostics

Dongkyu Lee
, Ohwon Kwon
, Kang Ho Lee
, Jongsu Yun
, Changwon kim
, Joonho Seo
, Jeawan Koo
, Gyu Man Kim

School of Mechanical Engineering

Korea Institute of Machinery and Materials
Kyungpook National University

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Fully automated system for sample preparation in molecular diagnostics was developed. The small cartridge with multiple chambers and a rotating valve was designed for automated processes such as extraction, separation and purifications during the DNA sample preparation. The automated processes such as injection, mixing, capturing and valve on/off motions were controlled by an affordable, motorized control system. We demonstrated the automated DNA extraction of pathogenic bacteria using this system. The deviations in the purity and real-time polymerase chain reaction (PCR) detection of the automated DNA extraction samples were clearly decreased. The device provided rapid, reliable, and reproducible sample preparation in comparison with manual DNA extraction. This automated platform can be utilized in emergent point-of-care testing of infectious disease and foodborne bacteria.

Original language	English
Pages (from-to)	288-293
Number of pages	6
Journal	Biochip Journal
Volume	13
Issue number	3
DOIs	https://doi.org/10.1007/s13206-019-3304-6
State	Published - 1 Sep 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Automated platform
Molecular diagnostics
Nucleic acids
Point-of-care testing
Sample preparation

Access to Document

10.1007/s13206-019-3304-6

Cite this

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title = "Automated Platform for Rapid and Reproducible Sample Preparations in Point-of-Care(POC) Molecular Diagnostics",

abstract = "Fully automated system for sample preparation in molecular diagnostics was developed. The small cartridge with multiple chambers and a rotating valve was designed for automated processes such as extraction, separation and purifications during the DNA sample preparation. The automated processes such as injection, mixing, capturing and valve on/off motions were controlled by an affordable, motorized control system. We demonstrated the automated DNA extraction of pathogenic bacteria using this system. The deviations in the purity and real-time polymerase chain reaction (PCR) detection of the automated DNA extraction samples were clearly decreased. The device provided rapid, reliable, and reproducible sample preparation in comparison with manual DNA extraction. This automated platform can be utilized in emergent point-of-care testing of infectious disease and foodborne bacteria.",

keywords = "Automated platform, Molecular diagnostics, Nucleic acids, Point-of-care testing, Sample preparation",

author = "Dongkyu Lee and Ohwon Kwon and Lee, \{Kang Ho\} and Jongsu Yun and Changwon kim and Joonho Seo and Jeawan Koo and Kim, \{Gyu Man\}",

note = "Publisher Copyright: {\textcopyright} 2019, The Korean BioChip Society and Springer.",

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doi = "10.1007/s13206-019-3304-6",

language = "English",

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AU - Lee, Dongkyu

AU - Kwon, Ohwon

AU - Lee, Kang Ho

AU - Yun, Jongsu

AU - kim, Changwon

AU - Seo, Joonho

AU - Koo, Jeawan

AU - Kim, Gyu Man

PY - 2019/9/1

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N2 - Fully automated system for sample preparation in molecular diagnostics was developed. The small cartridge with multiple chambers and a rotating valve was designed for automated processes such as extraction, separation and purifications during the DNA sample preparation. The automated processes such as injection, mixing, capturing and valve on/off motions were controlled by an affordable, motorized control system. We demonstrated the automated DNA extraction of pathogenic bacteria using this system. The deviations in the purity and real-time polymerase chain reaction (PCR) detection of the automated DNA extraction samples were clearly decreased. The device provided rapid, reliable, and reproducible sample preparation in comparison with manual DNA extraction. This automated platform can be utilized in emergent point-of-care testing of infectious disease and foodborne bacteria.

AB - Fully automated system for sample preparation in molecular diagnostics was developed. The small cartridge with multiple chambers and a rotating valve was designed for automated processes such as extraction, separation and purifications during the DNA sample preparation. The automated processes such as injection, mixing, capturing and valve on/off motions were controlled by an affordable, motorized control system. We demonstrated the automated DNA extraction of pathogenic bacteria using this system. The deviations in the purity and real-time polymerase chain reaction (PCR) detection of the automated DNA extraction samples were clearly decreased. The device provided rapid, reliable, and reproducible sample preparation in comparison with manual DNA extraction. This automated platform can be utilized in emergent point-of-care testing of infectious disease and foodborne bacteria.

KW - Automated platform

KW - Molecular diagnostics

KW - Nucleic acids

KW - Point-of-care testing

KW - Sample preparation

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ER -

Automated Platform for Rapid and Reproducible Sample Preparations in Point-of-Care(POC) Molecular Diagnostics

Abstract

UN SDGs

Keywords

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