TY - JOUR
T1 - Babesia gibsoni
T2 - Serodiagnosis of infection in dogs by an enzyme-linked immunosorbent assay with recombinant BgTRAP
AU - Goo, Youn Kyoung
AU - Jia, Honglin
AU - Aboge, G. Oluga
AU - Terkawi, M. Alaa
AU - Kuriki, Ken
AU - Nakamura, Chinatsu
AU - Kumagai, Akiko
AU - Zhou, Jinlin
AU - Lee, Eung goo
AU - Nishikawa, Yoshifumi
AU - Igarashi, Ikuo
AU - Fujisaki, Kozo
AU - Xuan, Xuenan
PY - 2008/4
Y1 - 2008/4
N2 - The thrombospondin-related adhesive protein of Babesia gibsoni (BgTRAP) is known as an immunodominant antigen and is, therefore, considered as a candidate for the development of a diagnostic reagent for canine babesiosis. The recombinant BgTRAP (rBgTRAP) expressed in Escherichia coli was tested in an enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to B. gibsoni in dogs. The ELISA with rBgTRAP clearly differentiated between B. gibsoni-infected dog sera and specific pathogen-free (SPF) dog sera. The sera collected from dogs experimentally infected with closely related parasites, B. canis canis, B. canis vogeli, B. canis rossi, and Neospora caninum, showed no cross-reactivity by the ELISA with rBgTRAP. A total of 107 blood samples collected from dogs that had been diagnosed as having babesiosis at veterinary hospitals in Japan were examined for the diagnosis of B. gibsoni infection by the ELISA and PCR. Ninety-six (89.7%) and 89 (83.2%) of the tested samples were positive by the ELISA and PCR, respectively, while 11 (10.3%) and 4 (3.7%) were ELISA+/PCR- and ELISA-/PCR+, respectively. In addition, the sensitivity of the ELISA with rBgTRAP was much higher than that of previously established ELISAs with rBgP50, rBgSA1, and rBgP32. These results indicate that the rBgTRAP is the most promising diagnostic antigen for the detection of an antibody to B. gibsoni in dogs and that the combined ELISA/PCR approach could provide the most reliable diagnosis for clinical sites.
AB - The thrombospondin-related adhesive protein of Babesia gibsoni (BgTRAP) is known as an immunodominant antigen and is, therefore, considered as a candidate for the development of a diagnostic reagent for canine babesiosis. The recombinant BgTRAP (rBgTRAP) expressed in Escherichia coli was tested in an enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to B. gibsoni in dogs. The ELISA with rBgTRAP clearly differentiated between B. gibsoni-infected dog sera and specific pathogen-free (SPF) dog sera. The sera collected from dogs experimentally infected with closely related parasites, B. canis canis, B. canis vogeli, B. canis rossi, and Neospora caninum, showed no cross-reactivity by the ELISA with rBgTRAP. A total of 107 blood samples collected from dogs that had been diagnosed as having babesiosis at veterinary hospitals in Japan were examined for the diagnosis of B. gibsoni infection by the ELISA and PCR. Ninety-six (89.7%) and 89 (83.2%) of the tested samples were positive by the ELISA and PCR, respectively, while 11 (10.3%) and 4 (3.7%) were ELISA+/PCR- and ELISA-/PCR+, respectively. In addition, the sensitivity of the ELISA with rBgTRAP was much higher than that of previously established ELISAs with rBgP50, rBgSA1, and rBgP32. These results indicate that the rBgTRAP is the most promising diagnostic antigen for the detection of an antibody to B. gibsoni in dogs and that the combined ELISA/PCR approach could provide the most reliable diagnosis for clinical sites.
KW - Babesia gibsoni
KW - Diagnosis
KW - Dogs
KW - ELISA
KW - enzyme-linked immunosorbent assay
KW - thrombospondin-related adhesive protein
KW - TRAP
UR - http://www.scopus.com/inward/record.url?scp=40649125051&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2007.11.010
DO - 10.1016/j.exppara.2007.11.010
M3 - Article
C2 - 18155197
AN - SCOPUS:40649125051
SN - 0014-4894
VL - 118
SP - 555
EP - 560
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 4
ER -
