TY - JOUR
T1 - Beneficial role of hydrogen sulfide in renal ischemia reperfusion injury in rats
AU - Choi, Eun Kyung
AU - Park, Sol Hee
AU - Lim, Jung A.
AU - Hong, Seong Wook
AU - Kwak, Kyung Hwa
AU - Park, Sung Sik
AU - Lim, Dong Gun
AU - Jung, Hoon
N1 - Publisher Copyright:
© Yonsei University College of Medicine 2018.
PY - 2018/10
Y1 - 2018/10
N2 - Purpose: Hydrogen sulfide (H2S) is an endogenous gaseous molecule with important physiological roles. It is synthesized from cysteine by cystathionine γ-lyase (CGL) and cystathionine β-synthase (CBS). The present study examined the benefits of exogenous H2S on renal ischemia reperfusion (IR) injury, as well as the effects of CGL or CBS inhibition. Furthermore, we elucidated the mechanism underlying the action of H2S in the kidneys. Materials and Methods: Thirty male Sprague-Dawley rats were randomly assigned to five groups: a sham, renal IR control, sodium hydrosulfide (NaHS) treatment, H2S donor, and CGL or CBS inhibitor administration group. Levels of blood urea nitrogen (BUN), serum creatinine (Cr), renal tissue malondialdehyde (MDA), and superoxide dismutase (SOD) were estimated. Histological changes, apoptosis, and expression of mitogen-activated protein kinase (MAPK) family members (extracellular signal-regu-lated kinase, c-Jun N-terminal kinase, and p38) were also evaluated. Results: NaHS attenuated serum BUN and Cr levels, as well as histological damage caused by renal IR injury. Administration of NaHS also reduced oxidative stress as evident from decreased MDA, preserved SOD, and reduced apoptotic cells. Additionally, NaHS prevented renal IR-induced MAPK phosphorylation. The CGL or CBS group showed increased MAPK family activity; however, there was no significant difference in the IR control group. Conclusion: Exogenous H2S can mitigate IR injury-led renal damage. The proposed beneficial effect of H2S is, in part, because of the anti-oxidative stress associated with modulation of the MAPK signaling pathways.
AB - Purpose: Hydrogen sulfide (H2S) is an endogenous gaseous molecule with important physiological roles. It is synthesized from cysteine by cystathionine γ-lyase (CGL) and cystathionine β-synthase (CBS). The present study examined the benefits of exogenous H2S on renal ischemia reperfusion (IR) injury, as well as the effects of CGL or CBS inhibition. Furthermore, we elucidated the mechanism underlying the action of H2S in the kidneys. Materials and Methods: Thirty male Sprague-Dawley rats were randomly assigned to five groups: a sham, renal IR control, sodium hydrosulfide (NaHS) treatment, H2S donor, and CGL or CBS inhibitor administration group. Levels of blood urea nitrogen (BUN), serum creatinine (Cr), renal tissue malondialdehyde (MDA), and superoxide dismutase (SOD) were estimated. Histological changes, apoptosis, and expression of mitogen-activated protein kinase (MAPK) family members (extracellular signal-regu-lated kinase, c-Jun N-terminal kinase, and p38) were also evaluated. Results: NaHS attenuated serum BUN and Cr levels, as well as histological damage caused by renal IR injury. Administration of NaHS also reduced oxidative stress as evident from decreased MDA, preserved SOD, and reduced apoptotic cells. Additionally, NaHS prevented renal IR-induced MAPK phosphorylation. The CGL or CBS group showed increased MAPK family activity; however, there was no significant difference in the IR control group. Conclusion: Exogenous H2S can mitigate IR injury-led renal damage. The proposed beneficial effect of H2S is, in part, because of the anti-oxidative stress associated with modulation of the MAPK signaling pathways.
KW - Cystathionine β-synthase
KW - Cystathionine γ-lyase
KW - Hydrogen sulfide
KW - Ischemia-reperfusion injury
UR - http://www.scopus.com/inward/record.url?scp=85054085535&partnerID=8YFLogxK
U2 - 10.3349/ymj.2018.59.8.960
DO - 10.3349/ymj.2018.59.8.960
M3 - Article
C2 - 30187703
AN - SCOPUS:85054085535
SN - 0513-5796
VL - 59
SP - 960
EP - 967
JO - Yonsei Medical Journal
JF - Yonsei Medical Journal
IS - 8
ER -