Bienzyme liquid-crystal-based cholesterol biosensor

Sundas Munir; Mashooq Khan; Soo Young Park

doi:10.1016/j.snb.2015.05.117

Bienzyme liquid-crystal-based cholesterol biosensor

Sundas Munir, Mashooq Khan, Soo Young Park

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

A cholesterol biosensor was fabricated by coating 4-cyano-4′-pentylbiphenyl (5CB) in a transmission electron microscope (TEM) grid with poly(acrylicacid-b-4-cyno-4′-undecyl acrylate) (PAA-b-LCP), followed by co-immobilization of cholesterol oxidase and horseradish peroxidase on the PAA brushes. This TEM grid sensor was tested for stability and sensitivity of the immobilized enzymes to cholesterol. Cholesterol was detected by a planar-to-homeotropic orientational change of 5CB at a limit of 0.8 mM. The high stability, ease of preparation, and specific detection of cholesterol in a complex mixture, could provide a new and convenient method for screening cholesterol levels in a sample using a polarized optical microscope.

Original language	English
Pages (from-to)	508-515
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	220
DOIs	https://doi.org/10.1016/j.snb.2015.05.117
State	Published - 22 Jun 2015

Keywords

5CB
Bienzymes
Block-copolymer
Peroxidase
TEM grid

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10.1016/j.snb.2015.05.117

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title = "Bienzyme liquid-crystal-based cholesterol biosensor",

abstract = "A cholesterol biosensor was fabricated by coating 4-cyano-4′-pentylbiphenyl (5CB) in a transmission electron microscope (TEM) grid with poly(acrylicacid-b-4-cyno-4′-undecyl acrylate) (PAA-b-LCP), followed by co-immobilization of cholesterol oxidase and horseradish peroxidase on the PAA brushes. This TEM grid sensor was tested for stability and sensitivity of the immobilized enzymes to cholesterol. Cholesterol was detected by a planar-to-homeotropic orientational change of 5CB at a limit of 0.8 mM. The high stability, ease of preparation, and specific detection of cholesterol in a complex mixture, could provide a new and convenient method for screening cholesterol levels in a sample using a polarized optical microscope.",

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T1 - Bienzyme liquid-crystal-based cholesterol biosensor

AU - Munir, Sundas

AU - Khan, Mashooq

AU - Park, Soo Young

PY - 2015/6/22

Y1 - 2015/6/22

N2 - A cholesterol biosensor was fabricated by coating 4-cyano-4′-pentylbiphenyl (5CB) in a transmission electron microscope (TEM) grid with poly(acrylicacid-b-4-cyno-4′-undecyl acrylate) (PAA-b-LCP), followed by co-immobilization of cholesterol oxidase and horseradish peroxidase on the PAA brushes. This TEM grid sensor was tested for stability and sensitivity of the immobilized enzymes to cholesterol. Cholesterol was detected by a planar-to-homeotropic orientational change of 5CB at a limit of 0.8 mM. The high stability, ease of preparation, and specific detection of cholesterol in a complex mixture, could provide a new and convenient method for screening cholesterol levels in a sample using a polarized optical microscope.

AB - A cholesterol biosensor was fabricated by coating 4-cyano-4′-pentylbiphenyl (5CB) in a transmission electron microscope (TEM) grid with poly(acrylicacid-b-4-cyno-4′-undecyl acrylate) (PAA-b-LCP), followed by co-immobilization of cholesterol oxidase and horseradish peroxidase on the PAA brushes. This TEM grid sensor was tested for stability and sensitivity of the immobilized enzymes to cholesterol. Cholesterol was detected by a planar-to-homeotropic orientational change of 5CB at a limit of 0.8 mM. The high stability, ease of preparation, and specific detection of cholesterol in a complex mixture, could provide a new and convenient method for screening cholesterol levels in a sample using a polarized optical microscope.

KW - 5CB

KW - Bienzymes

KW - Block-copolymer

KW - Peroxidase

KW - TEM grid

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JO - Sensors and Actuators, B: Chemical

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Bienzyme liquid-crystal-based cholesterol biosensor

Abstract

Keywords

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