Binding mode of [ruthenium(II)(1,10-phenanthroline)2L]2+ to poly(dG)·poly(dC)·poly(dC)+ triplex DNA

C. B. Cho; T. S. Cho; S. K. Kim; B. J. Kim; S. W. Han; M. J. Jung

Binding mode of [ruthenium(II)(1,10-phenanthroline)₂L]²⁺ to poly(dG)·poly(dC)·poly(dC)⁺ triplex DNA

C. B. Cho, T. S. Cho, S. K. Kim, B. J. Kim, S. W. Han, M. J. Jung

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Binding geometries of [Ru(II)(1,10-phenanthroline)₂L]²⁺ complexes (where L = dipyrido [3,2-a:2',3'-c]phenazine (DPPZ) or benzodipyrido[3,2-a:2',3'-c]phenazine (BDPPZ)) to poly(dG)·poly(dC)·poly(dC)⁺ triplex DNA (CGC⁺ triplex) has been investigated by linear dichroism and normal absorption spectroscopy. Analysis of the linear dichroism for the CGC⁺ triplex and [Ru(II)(phen)₂BDPPZ]²⁺ complex indicates that the extended ligand of the metal complex lie perpendicular to the polynucleotide helix axis. Together with strong hypochromism and red shift in the interligand absorption region, we concluded that the extended BDPPZ or DPPZ ligand intercalated between the bases of polynucleotide. The spectral properties of the metal complexes bound to CGC⁺ triplex are similar to those bound to poly(dA)[poly(dT)]₂ triplex (Choi et al., Biochemistry 1997, 36, 214), suggesting that the metal complex is located in the minor groove of the CGC⁺ triplex.

Original language	English
Pages (from-to)	995-999
Number of pages	5
Journal	Bulletin of the Korean Chemical Society
Volume	21
Issue number	10
State	Published - 20 Oct 2000

Cite this

@article{3f3d048d8c1443f9aa59842c6bb5281e,

title = "Binding mode of [ruthenium(II)(1,10-phenanthroline)2L]2+ to poly(dG)·poly(dC)·poly(dC)+ triplex DNA",

abstract = "Binding geometries of [Ru(II)(1,10-phenanthroline)2L]2+ complexes (where L = dipyrido [3,2-a:2',3'-c]phenazine (DPPZ) or benzodipyrido[3,2-a:2',3'-c]phenazine (BDPPZ)) to poly(dG)·poly(dC)·poly(dC)+ triplex DNA (CGC+ triplex) has been investigated by linear dichroism and normal absorption spectroscopy. Analysis of the linear dichroism for the CGC+ triplex and [Ru(II)(phen)2BDPPZ]2+ complex indicates that the extended ligand of the metal complex lie perpendicular to the polynucleotide helix axis. Together with strong hypochromism and red shift in the interligand absorption region, we concluded that the extended BDPPZ or DPPZ ligand intercalated between the bases of polynucleotide. The spectral properties of the metal complexes bound to CGC+ triplex are similar to those bound to poly(dA)[poly(dT)]2 triplex (Choi et al., Biochemistry 1997, 36, 214), suggesting that the metal complex is located in the minor groove of the CGC+ triplex.",

author = "Cho, \{C. B.\} and Cho, \{T. S.\} and Kim, \{S. K.\} and Kim, \{B. J.\} and Han, \{S. W.\} and Jung, \{M. J.\}",

year = "2000",

month = oct,

day = "20",

language = "English",

volume = "21",

pages = "995--999",

journal = "Bulletin of the Korean Chemical Society",

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TY - JOUR

T1 - Binding mode of [ruthenium(II)(1,10-phenanthroline)2L]2+ to poly(dG)·poly(dC)·poly(dC)+ triplex DNA

AU - Cho, C. B.

AU - Cho, T. S.

AU - Kim, S. K.

AU - Kim, B. J.

AU - Han, S. W.

AU - Jung, M. J.

PY - 2000/10/20

Y1 - 2000/10/20

N2 - Binding geometries of [Ru(II)(1,10-phenanthroline)2L]2+ complexes (where L = dipyrido [3,2-a:2',3'-c]phenazine (DPPZ) or benzodipyrido[3,2-a:2',3'-c]phenazine (BDPPZ)) to poly(dG)·poly(dC)·poly(dC)+ triplex DNA (CGC+ triplex) has been investigated by linear dichroism and normal absorption spectroscopy. Analysis of the linear dichroism for the CGC+ triplex and [Ru(II)(phen)2BDPPZ]2+ complex indicates that the extended ligand of the metal complex lie perpendicular to the polynucleotide helix axis. Together with strong hypochromism and red shift in the interligand absorption region, we concluded that the extended BDPPZ or DPPZ ligand intercalated between the bases of polynucleotide. The spectral properties of the metal complexes bound to CGC+ triplex are similar to those bound to poly(dA)[poly(dT)]2 triplex (Choi et al., Biochemistry 1997, 36, 214), suggesting that the metal complex is located in the minor groove of the CGC+ triplex.

AB - Binding geometries of [Ru(II)(1,10-phenanthroline)2L]2+ complexes (where L = dipyrido [3,2-a:2',3'-c]phenazine (DPPZ) or benzodipyrido[3,2-a:2',3'-c]phenazine (BDPPZ)) to poly(dG)·poly(dC)·poly(dC)+ triplex DNA (CGC+ triplex) has been investigated by linear dichroism and normal absorption spectroscopy. Analysis of the linear dichroism for the CGC+ triplex and [Ru(II)(phen)2BDPPZ]2+ complex indicates that the extended ligand of the metal complex lie perpendicular to the polynucleotide helix axis. Together with strong hypochromism and red shift in the interligand absorption region, we concluded that the extended BDPPZ or DPPZ ligand intercalated between the bases of polynucleotide. The spectral properties of the metal complexes bound to CGC+ triplex are similar to those bound to poly(dA)[poly(dT)]2 triplex (Choi et al., Biochemistry 1997, 36, 214), suggesting that the metal complex is located in the minor groove of the CGC+ triplex.

UR - https://www.scopus.com/pages/publications/0034692854

M3 - Article

AN - SCOPUS:0034692854

SN - 0253-2964

VL - 21

SP - 995

EP - 999

JO - Bulletin of the Korean Chemical Society

JF - Bulletin of the Korean Chemical Society

IS - 10

ER -

Binding mode of [ruthenium(II)(1,10-phenanthroline)₂L]²⁺ to poly(dG)·poly(dC)·poly(dC)⁺ triplex DNA

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