Bioassay-guided isolation and identification of anti-platelet-active compounds from the root of Ashitaba (Angelica keiskei Koidz.)

Dong Ju Son, Ye Oak Park, Chengguang Yu, Sung Eun Lee, Young Hyun Park

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Platelet aggregation is fundamental to a wide range of physiological and pathological processes, including the induction of thrombosis and arteriosclerosis. Anti-platelet activity of a crude methanol extract and solvent fractions of Ashitaba roots (Angelica keiskei Koidz.) was evaluated using a turbidimetric method using washed rabbit platelets. We identified the anti-platelet activities of two chalcones, 4-hydroxyderricin and xanthoangelol, isolated from the ethyl acetate-soluble fraction of Ashitaba roots by using a bioassay-guided isolation method. 4-Hydroxyderricin and xanthoangelol effectively inhibited platelet aggregation induced by collagen (IC50 of 41.9 and 35.9 M, respectively), platelet-activating factor (IC50 of 46.1 and 42.3 M, respectively) and phorbol 12-myristate 13-acetate (IC50 of 16.5 and 45.9 M, respectively). These compounds did not inhibit thrombin-induced platelet aggregation (IC50 of>80 M). The results suggest that the chalcones 4-hydroxyderricin and xanthoangelol may be potent anti-thrombotic components of A. keiskei Koidz.

Original languageEnglish
Pages (from-to)2312-2316
Number of pages5
JournalNatural Product Research
Volume28
Issue number24
DOIs
StatePublished - 17 Dec 2014

Keywords

  • 4-hydroxyderricin
  • Angelica keiskei Koidaz
  • anti-platelet
  • Ashitaba
  • xanthoangelol

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