Biodegradation of dibutylphthalate by white rot fungi and evaluation on its estrogenic activity

Soo Min Lee, Bon Wook Koo, Sung Suk Lee, Myung Kil Kim, Don Ha Choi, Eui Ju Hong, Eui Bae Jeung, In Gyu Choi

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50 Scopus citations

Abstract

The biodegradation and estrogenic activity of dibutylphthalate (DBP) were characterized in the white rot fungi, Phanerochaete chrysosporium, Trametes versicolor and Daldinia concentrica. The DBP resistance of these fungi was determined in a 2% malt extract agar medium. The biodegradation efficiency was tested in the concentration of 100 μM DBP, its degraded products were analyzed by HPLC and GC/MS, and the induction effect by DBP was measured by laccase and manganese peroxidase. The reduction of estrogenic effect was assayed by MCF-7 cell proliferation test and measurement of pS2 mRNA expression. Although the mycelium growth rate of D. concentrica was slow in the media containing DBP, its resistance to DBP was at the high concentration of 500 ppm. However, at the low concentration less than 100 ppm of DBP, the mycelium growth rates of P. chrysosporium and T. versicolor were superior to D. concentrica. The induction of laccase was effective in T. versicolor, but not in D. concentrica and P. chyrysosporium. The degradation efficiency was 94% in D. concentrica, 83% in T. versicolor after first day of incubation, and completely degraded after 6 days of incubation. However, proliferation of the cell lines was significantly blocked when the cells were incubated in the DBP culture medium of D. concentrica after 6 days of incubation. The levels of pS2 mRNA expression were decreased in supernatant from DBP treated with D. concentrica compared with DBP only treatment after one day of incubation.

Original languageEnglish
Pages (from-to)417-423
Number of pages7
JournalEnzyme and Microbial Technology
Volume35
Issue number5
DOIs
StatePublished - 6 Oct 2004

Keywords

  • Biodegradation
  • Daldinia concentrica
  • Dibutylphthalate
  • Estrogenic activity
  • White rot fungi

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