Biological hydrogen production by immobilized cells of Clostridium tyrobutyricum JM1 isolated from a food waste treatment process

Ji Hye Jo, Dae Sung Lee, Donghee Park, Jong Moon Park

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131 Scopus citations

Abstract

A fermentative hydrogen-producing bacterium, Clostridium tyrobutyricum JM1, was isolated from a food waste treating process using 16S rRNA gene sequencing and amplified ribosomal DNA restriction analysis (ARDRA). A fixed-bed bioreactor packed with polyurethane foam as support matrix for the growth of the isolate was operated at different hydraulic retention time (HRT) to evaluate its performance for hydrogen production. The reactor achieved the maximal hydrogen production rate of 7.2 l H2 l-1 d-1 at 2 h HRT, where hydrogen content in biogas was 50.0%, and substrate conversion efficiency was 97.4%. The maximum hydrogen yield was 223 ml (g-hexose)-1 with an influent glucose concentration of 5 g l-1. Therefore, the immobilized reactor using C. tyrobutyricum JM1 was an effective and stable system for continuous hydrogen production.

Original languageEnglish
Pages (from-to)6666-6672
Number of pages7
JournalBioresource Technology
Volume99
Issue number14
DOIs
StatePublished - Sep 2008

Keywords

  • ARDRA
  • Clostridium tyrobutyricum
  • Food waste
  • Hydrogen production
  • Immobilized bioreactor

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