BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2

Mi Hye Lee; Tae Geon Kwon; Hyo Sang Park; John M. Wozney; Hyun Mo Ryoo

doi:10.1016/j.bbrc.2003.08.058

BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2

Mi Hye Lee, Tae Geon Kwon, Hyo Sang Park, John M. Wozney, Hyun Mo Ryoo

Department of Dentistry

Research output: Contribution to journal › Article › peer-review

363 Scopus citations

Abstract

BMP-2 stimulates the expression of three osteogenic master transcription factors: Runx2, Dlx5, and Osterix (Osx). However, the hierarchical regulatory relationships among them are not yet clearly understood. Osx was commonly stimulated in osteogenic and non-osteogenic cells in response to BMP-signaling, as Dlx5 was in our previous report. A cycloheximide experiment indicated that Osx expression by BMP-2 requires new protein synthesis. Even if Osx has been suggested as a downstream target of Runx2, the results of this study indicated that Osx expression was still induced by BMP-2 treatment in Runx2 null cells, but not induced by Runx2 overexpression in myogenic C2C12 cells. Instead, Osx expression by BMP-2 was completely abrogated by the antisense blocking of Dlx5. Depending upon the coincident expression pattern of Osx and Dlx5, and the blocking of Osx expression by the antisense Dlx5, BMP-2-induced Osx expression is mainly mediated not by Runx2, but by Dlx5.

Original language	English
Pages (from-to)	689-694
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	309
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2003.08.058
State	Published - 26 Sep 2003

Keywords

BMP-2
Dlx5
Osteoblast
Osterix
Runx2

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@article{e20dab43fd474f80a99eaddd66e28845,

title = "BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2",

abstract = "BMP-2 stimulates the expression of three osteogenic master transcription factors: Runx2, Dlx5, and Osterix (Osx). However, the hierarchical regulatory relationships among them are not yet clearly understood. Osx was commonly stimulated in osteogenic and non-osteogenic cells in response to BMP-signaling, as Dlx5 was in our previous report. A cycloheximide experiment indicated that Osx expression by BMP-2 requires new protein synthesis. Even if Osx has been suggested as a downstream target of Runx2, the results of this study indicated that Osx expression was still induced by BMP-2 treatment in Runx2 null cells, but not induced by Runx2 overexpression in myogenic C2C12 cells. Instead, Osx expression by BMP-2 was completely abrogated by the antisense blocking of Dlx5. Depending upon the coincident expression pattern of Osx and Dlx5, and the blocking of Osx expression by the antisense Dlx5, BMP-2-induced Osx expression is mainly mediated not by Runx2, but by Dlx5.",

keywords = "BMP-2, Dlx5, Osteoblast, Osterix, Runx2",

author = "Lee, \{Mi Hye\} and Kwon, \{Tae Geon\} and Park, \{Hyo Sang\} and Wozney, \{John M.\} and Ryoo, \{Hyun Mo\}",

year = "2003",

month = sep,

day = "26",

doi = "10.1016/j.bbrc.2003.08.058",

language = "English",

volume = "309",

pages = "689--694",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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TY - JOUR

T1 - BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2

AU - Lee, Mi Hye

AU - Kwon, Tae Geon

AU - Park, Hyo Sang

AU - Wozney, John M.

AU - Ryoo, Hyun Mo

PY - 2003/9/26

Y1 - 2003/9/26

N2 - BMP-2 stimulates the expression of three osteogenic master transcription factors: Runx2, Dlx5, and Osterix (Osx). However, the hierarchical regulatory relationships among them are not yet clearly understood. Osx was commonly stimulated in osteogenic and non-osteogenic cells in response to BMP-signaling, as Dlx5 was in our previous report. A cycloheximide experiment indicated that Osx expression by BMP-2 requires new protein synthesis. Even if Osx has been suggested as a downstream target of Runx2, the results of this study indicated that Osx expression was still induced by BMP-2 treatment in Runx2 null cells, but not induced by Runx2 overexpression in myogenic C2C12 cells. Instead, Osx expression by BMP-2 was completely abrogated by the antisense blocking of Dlx5. Depending upon the coincident expression pattern of Osx and Dlx5, and the blocking of Osx expression by the antisense Dlx5, BMP-2-induced Osx expression is mainly mediated not by Runx2, but by Dlx5.

AB - BMP-2 stimulates the expression of three osteogenic master transcription factors: Runx2, Dlx5, and Osterix (Osx). However, the hierarchical regulatory relationships among them are not yet clearly understood. Osx was commonly stimulated in osteogenic and non-osteogenic cells in response to BMP-signaling, as Dlx5 was in our previous report. A cycloheximide experiment indicated that Osx expression by BMP-2 requires new protein synthesis. Even if Osx has been suggested as a downstream target of Runx2, the results of this study indicated that Osx expression was still induced by BMP-2 treatment in Runx2 null cells, but not induced by Runx2 overexpression in myogenic C2C12 cells. Instead, Osx expression by BMP-2 was completely abrogated by the antisense blocking of Dlx5. Depending upon the coincident expression pattern of Osx and Dlx5, and the blocking of Osx expression by the antisense Dlx5, BMP-2-induced Osx expression is mainly mediated not by Runx2, but by Dlx5.

KW - BMP-2

KW - Dlx5

KW - Osteoblast

KW - Osterix

KW - Runx2

UR - https://www.scopus.com/pages/publications/0041328856

U2 - 10.1016/j.bbrc.2003.08.058

DO - 10.1016/j.bbrc.2003.08.058

M3 - Article

C2 - 12963046

AN - SCOPUS:0041328856

SN - 0006-291X

VL - 309

SP - 689

EP - 694

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2

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