Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd2O3 nanoparticles: Synthesis, characterization, and application to MRI contrast agents

Md Wasi Ahmad; Cho Rong Kim; Jong Su Baeck; Yongmin Chang; Tae Jeong Kim; Ji Eun Bae; Kwon Seok Chae; Gang Ho Lee

doi:10.1016/j.colsurfa.2014.03.011

Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd₂O₃ nanoparticles: Synthesis, characterization, and application to MRI contrast agents

Md Wasi Ahmad, Cho Rong Kim, Jong Su Baeck, Yongmin Chang, Tae Jeong Kim, Ji Eun Bae, Kwon Seok Chae, Gang Ho Lee

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Bovine serum albumin (BSA) (M_n=66.5kD, size=14×4×4nm) is an attractive biological molecule for biomedical applications because of its water-solubility and bio-compatibility. It can also bind many ultrasmall nanoparticles (NPs) as confirmed in this study. We synthesized polyethylene glycol diacid (PEGD) coated ultrasmall Gd₂O₃ nanoparticles (PEGD-GNPs, the core d_avg=2.0nm), which were then conjugated to BSA and cleaved-BSA (C-BSA) (i.e. BSA-PEGD-GNPs and C-BSA-PEGD-GNPs) through amide bonding. Large relaxivities were observed in both aqueous sample solutions (r₁=6.0s^-1mM^-1 and r₂=28.0s^-1mM^-1 for BSA-PEGD-GNPs and r₁=7.6s^-1mM^-1 and r₂=22.0s^-1mM^-1 for C-BSA-PEGD-GNPs). Three tesla T₂ magnetic resonance imaging (MRI) in a mouse after the injection of an aqueous sample solution of BSA-PEGD-GNPs into a mouse tail vein revealed significant negative contrast enhancements. Large relaxivities and in vivo MR images prove that BSA-PEGD-GNPs and C-BSA-PEGD-GNPs are potential MRI contrast agents.

Original language	English
Pages (from-to)	67-75
Number of pages	9
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	450
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfa.2014.03.011
State	Published - 20 May 2014

Keywords

BSA
C-BSA
Contrast agent
MRI
Nanoparticle carrier
Ultrasmall GdO nanoparticle

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10.1016/j.colsurfa.2014.03.011

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Ahmad, M. W., Kim, C. R., Baeck, J. S., Chang, Y., Kim, T. J., Bae, J. E., Chae, K. S., & Lee, G. H. (2014). Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd₂O₃ nanoparticles: Synthesis, characterization, and application to MRI contrast agents. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 450(1), 67-75. https://doi.org/10.1016/j.colsurfa.2014.03.011

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title = "Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd2O3 nanoparticles: Synthesis, characterization, and application to MRI contrast agents",

abstract = "Bovine serum albumin (BSA) (Mn=66.5kD, size=14×4×4nm) is an attractive biological molecule for biomedical applications because of its water-solubility and bio-compatibility. It can also bind many ultrasmall nanoparticles (NPs) as confirmed in this study. We synthesized polyethylene glycol diacid (PEGD) coated ultrasmall Gd2O3 nanoparticles (PEGD-GNPs, the core davg=2.0nm), which were then conjugated to BSA and cleaved-BSA (C-BSA) (i.e. BSA-PEGD-GNPs and C-BSA-PEGD-GNPs) through amide bonding. Large relaxivities were observed in both aqueous sample solutions (r1=6.0s-1mM-1 and r2=28.0s-1mM-1 for BSA-PEGD-GNPs and r1=7.6s-1mM-1 and r2=22.0s-1mM-1 for C-BSA-PEGD-GNPs). Three tesla T2 magnetic resonance imaging (MRI) in a mouse after the injection of an aqueous sample solution of BSA-PEGD-GNPs into a mouse tail vein revealed significant negative contrast enhancements. Large relaxivities and in vivo MR images prove that BSA-PEGD-GNPs and C-BSA-PEGD-GNPs are potential MRI contrast agents.",

keywords = "BSA, C-BSA, Contrast agent, MRI, Nanoparticle carrier, Ultrasmall GdO nanoparticle",

author = "Ahmad, {Md Wasi} and Kim, {Cho Rong} and Baeck, {Jong Su} and Yongmin Chang and Kim, {Tae Jeong} and Bae, {Ji Eun} and Chae, {Kwon Seok} and Lee, {Gang Ho}",

year = "2014",

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doi = "10.1016/j.colsurfa.2014.03.011",

language = "English",

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Ahmad, MW, Kim, CR, Baeck, JS, Chang, Y, Kim, TJ, Bae, JE, Chae, KS & Lee, GH 2014, 'Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd₂O₃ nanoparticles: Synthesis, characterization, and application to MRI contrast agents', Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 450, no. 1, pp. 67-75. https://doi.org/10.1016/j.colsurfa.2014.03.011

Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd₂O₃ nanoparticles: Synthesis, characterization, and application to MRI contrast agents. / Ahmad, Md Wasi; Kim, Cho Rong; Baeck, Jong Su et al.
In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 450, No. 1, 20.05.2014, p. 67-75.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd2O3 nanoparticles

T2 - Synthesis, characterization, and application to MRI contrast agents

AU - Ahmad, Md Wasi

AU - Kim, Cho Rong

AU - Baeck, Jong Su

AU - Chang, Yongmin

AU - Kim, Tae Jeong

AU - Bae, Ji Eun

AU - Chae, Kwon Seok

AU - Lee, Gang Ho

PY - 2014/5/20

Y1 - 2014/5/20

N2 - Bovine serum albumin (BSA) (Mn=66.5kD, size=14×4×4nm) is an attractive biological molecule for biomedical applications because of its water-solubility and bio-compatibility. It can also bind many ultrasmall nanoparticles (NPs) as confirmed in this study. We synthesized polyethylene glycol diacid (PEGD) coated ultrasmall Gd2O3 nanoparticles (PEGD-GNPs, the core davg=2.0nm), which were then conjugated to BSA and cleaved-BSA (C-BSA) (i.e. BSA-PEGD-GNPs and C-BSA-PEGD-GNPs) through amide bonding. Large relaxivities were observed in both aqueous sample solutions (r1=6.0s-1mM-1 and r2=28.0s-1mM-1 for BSA-PEGD-GNPs and r1=7.6s-1mM-1 and r2=22.0s-1mM-1 for C-BSA-PEGD-GNPs). Three tesla T2 magnetic resonance imaging (MRI) in a mouse after the injection of an aqueous sample solution of BSA-PEGD-GNPs into a mouse tail vein revealed significant negative contrast enhancements. Large relaxivities and in vivo MR images prove that BSA-PEGD-GNPs and C-BSA-PEGD-GNPs are potential MRI contrast agents.

AB - Bovine serum albumin (BSA) (Mn=66.5kD, size=14×4×4nm) is an attractive biological molecule for biomedical applications because of its water-solubility and bio-compatibility. It can also bind many ultrasmall nanoparticles (NPs) as confirmed in this study. We synthesized polyethylene glycol diacid (PEGD) coated ultrasmall Gd2O3 nanoparticles (PEGD-GNPs, the core davg=2.0nm), which were then conjugated to BSA and cleaved-BSA (C-BSA) (i.e. BSA-PEGD-GNPs and C-BSA-PEGD-GNPs) through amide bonding. Large relaxivities were observed in both aqueous sample solutions (r1=6.0s-1mM-1 and r2=28.0s-1mM-1 for BSA-PEGD-GNPs and r1=7.6s-1mM-1 and r2=22.0s-1mM-1 for C-BSA-PEGD-GNPs). Three tesla T2 magnetic resonance imaging (MRI) in a mouse after the injection of an aqueous sample solution of BSA-PEGD-GNPs into a mouse tail vein revealed significant negative contrast enhancements. Large relaxivities and in vivo MR images prove that BSA-PEGD-GNPs and C-BSA-PEGD-GNPs are potential MRI contrast agents.

KW - BSA

KW - C-BSA

KW - Contrast agent

KW - MRI

KW - Nanoparticle carrier

KW - Ultrasmall GdO nanoparticle

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VL - 450

SP - 67

EP - 75

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

IS - 1

ER -

Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd₂O₃ nanoparticles: Synthesis, characterization, and application to MRI contrast agents

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Keywords

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