Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd2O3 nanoparticles: Synthesis, characterization, and application to MRI contrast agents

Md Wasi Ahmad; Cho Rong Kim; Jong Su Baeck; Yongmin Chang; Tae Jeong Kim; Ji Eun Bae; Kwon Seok Chae; Gang Ho Lee

doi:10.1016/j.colsurfa.2014.03.011

Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd₂O₃ nanoparticles: Synthesis, characterization, and application to MRI contrast agents

Md Wasi Ahmad
, Cho Rong Kim
, Jong Su Baeck
, Yongmin Chang
, Tae Jeong Kim
, Ji Eun Bae
, Kwon Seok Chae
, Gang Ho Lee

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Bovine serum albumin (BSA) (M_n=66.5kD, size=14×4×4nm) is an attractive biological molecule for biomedical applications because of its water-solubility and bio-compatibility. It can also bind many ultrasmall nanoparticles (NPs) as confirmed in this study. We synthesized polyethylene glycol diacid (PEGD) coated ultrasmall Gd₂O₃ nanoparticles (PEGD-GNPs, the core d_avg=2.0nm), which were then conjugated to BSA and cleaved-BSA (C-BSA) (i.e. BSA-PEGD-GNPs and C-BSA-PEGD-GNPs) through amide bonding. Large relaxivities were observed in both aqueous sample solutions (r₁=6.0s^-1mM^-1 and r₂=28.0s^-1mM^-1 for BSA-PEGD-GNPs and r₁=7.6s^-1mM^-1 and r₂=22.0s^-1mM^-1 for C-BSA-PEGD-GNPs). Three tesla T₂ magnetic resonance imaging (MRI) in a mouse after the injection of an aqueous sample solution of BSA-PEGD-GNPs into a mouse tail vein revealed significant negative contrast enhancements. Large relaxivities and in vivo MR images prove that BSA-PEGD-GNPs and C-BSA-PEGD-GNPs are potential MRI contrast agents.

Original language	English
Pages (from-to)	67-75
Number of pages	9
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	450
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfa.2014.03.011
State	Published - 20 May 2014

Keywords

BSA
C-BSA
Contrast agent
MRI
Nanoparticle carrier
Ultrasmall GdO nanoparticle

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10.1016/j.colsurfa.2014.03.011

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Ahmad, M. W., Kim, C. R., Baeck, J. S., Chang, Y., Kim, T. J., Bae, J. E., Chae, K. S., & Lee, G. H. (2014). Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd₂O₃ nanoparticles: Synthesis, characterization, and application to MRI contrast agents. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 450(1), 67-75. https://doi.org/10.1016/j.colsurfa.2014.03.011

@article{f2c94f69abc94378a2faf5b01c9467de,

title = "Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd2O3 nanoparticles: Synthesis, characterization, and application to MRI contrast agents",

abstract = "Bovine serum albumin (BSA) (Mn=66.5kD, size=14×4×4nm) is an attractive biological molecule for biomedical applications because of its water-solubility and bio-compatibility. It can also bind many ultrasmall nanoparticles (NPs) as confirmed in this study. We synthesized polyethylene glycol diacid (PEGD) coated ultrasmall Gd2O3 nanoparticles (PEGD-GNPs, the core davg=2.0nm), which were then conjugated to BSA and cleaved-BSA (C-BSA) (i.e. BSA-PEGD-GNPs and C-BSA-PEGD-GNPs) through amide bonding. Large relaxivities were observed in both aqueous sample solutions (r1=6.0s-1mM-1 and r2=28.0s-1mM-1 for BSA-PEGD-GNPs and r1=7.6s-1mM-1 and r2=22.0s-1mM-1 for C-BSA-PEGD-GNPs). Three tesla T2 magnetic resonance imaging (MRI) in a mouse after the injection of an aqueous sample solution of BSA-PEGD-GNPs into a mouse tail vein revealed significant negative contrast enhancements. Large relaxivities and in vivo MR images prove that BSA-PEGD-GNPs and C-BSA-PEGD-GNPs are potential MRI contrast agents.",

keywords = "BSA, C-BSA, Contrast agent, MRI, Nanoparticle carrier, Ultrasmall GdO nanoparticle",

author = "Ahmad, \{Md Wasi\} and Kim, \{Cho Rong\} and Baeck, \{Jong Su\} and Yongmin Chang and Kim, \{Tae Jeong\} and Bae, \{Ji Eun\} and Chae, \{Kwon Seok\} and Lee, \{Gang Ho\}",

year = "2014",

month = may,

day = "20",

doi = "10.1016/j.colsurfa.2014.03.011",

language = "English",

volume = "450",

pages = "67--75",

journal = "Colloids and Surfaces A: Physicochemical and Engineering Aspects",

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Ahmad, MW, Kim, CR, Baeck, JS, Chang, Y, Kim, TJ, Bae, JE, Chae, KS & Lee, GH 2014, 'Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd₂O₃ nanoparticles: Synthesis, characterization, and application to MRI contrast agents', Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 450, no. 1, pp. 67-75. https://doi.org/10.1016/j.colsurfa.2014.03.011

Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd₂O₃ nanoparticles: Synthesis, characterization, and application to MRI contrast agents. / Ahmad, Md Wasi; Kim, Cho Rong; Baeck, Jong Su et al.
In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 450, No. 1, 20.05.2014, p. 67-75.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd2O3 nanoparticles

T2 - Synthesis, characterization, and application to MRI contrast agents

AU - Ahmad, Md Wasi

AU - Kim, Cho Rong

AU - Baeck, Jong Su

AU - Chang, Yongmin

AU - Kim, Tae Jeong

AU - Bae, Ji Eun

AU - Chae, Kwon Seok

AU - Lee, Gang Ho

PY - 2014/5/20

Y1 - 2014/5/20

N2 - Bovine serum albumin (BSA) (Mn=66.5kD, size=14×4×4nm) is an attractive biological molecule for biomedical applications because of its water-solubility and bio-compatibility. It can also bind many ultrasmall nanoparticles (NPs) as confirmed in this study. We synthesized polyethylene glycol diacid (PEGD) coated ultrasmall Gd2O3 nanoparticles (PEGD-GNPs, the core davg=2.0nm), which were then conjugated to BSA and cleaved-BSA (C-BSA) (i.e. BSA-PEGD-GNPs and C-BSA-PEGD-GNPs) through amide bonding. Large relaxivities were observed in both aqueous sample solutions (r1=6.0s-1mM-1 and r2=28.0s-1mM-1 for BSA-PEGD-GNPs and r1=7.6s-1mM-1 and r2=22.0s-1mM-1 for C-BSA-PEGD-GNPs). Three tesla T2 magnetic resonance imaging (MRI) in a mouse after the injection of an aqueous sample solution of BSA-PEGD-GNPs into a mouse tail vein revealed significant negative contrast enhancements. Large relaxivities and in vivo MR images prove that BSA-PEGD-GNPs and C-BSA-PEGD-GNPs are potential MRI contrast agents.

AB - Bovine serum albumin (BSA) (Mn=66.5kD, size=14×4×4nm) is an attractive biological molecule for biomedical applications because of its water-solubility and bio-compatibility. It can also bind many ultrasmall nanoparticles (NPs) as confirmed in this study. We synthesized polyethylene glycol diacid (PEGD) coated ultrasmall Gd2O3 nanoparticles (PEGD-GNPs, the core davg=2.0nm), which were then conjugated to BSA and cleaved-BSA (C-BSA) (i.e. BSA-PEGD-GNPs and C-BSA-PEGD-GNPs) through amide bonding. Large relaxivities were observed in both aqueous sample solutions (r1=6.0s-1mM-1 and r2=28.0s-1mM-1 for BSA-PEGD-GNPs and r1=7.6s-1mM-1 and r2=22.0s-1mM-1 for C-BSA-PEGD-GNPs). Three tesla T2 magnetic resonance imaging (MRI) in a mouse after the injection of an aqueous sample solution of BSA-PEGD-GNPs into a mouse tail vein revealed significant negative contrast enhancements. Large relaxivities and in vivo MR images prove that BSA-PEGD-GNPs and C-BSA-PEGD-GNPs are potential MRI contrast agents.

KW - BSA

KW - C-BSA

KW - Contrast agent

KW - MRI

KW - Nanoparticle carrier

KW - Ultrasmall GdO nanoparticle

UR - https://www.scopus.com/pages/publications/84897862379

U2 - 10.1016/j.colsurfa.2014.03.011

DO - 10.1016/j.colsurfa.2014.03.011

M3 - Article

AN - SCOPUS:84897862379

SN - 0927-7757

VL - 450

SP - 67

EP - 75

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

IS - 1

ER -

Bovine serum albumin (BSA) and cleaved-BSA conjugated ultrasmall Gd₂O₃ nanoparticles: Synthesis, characterization, and application to MRI contrast agents

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Keywords

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