C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses

Mohamad Alaa Terkawi; Hend Alhasan; Akio Ueno; Jadsada Ratthanophart; Yuzi Luo; Shinuo Cao; Ketsarin Kamyingkird; Mahmoud Aboulaila; Goo Youn-Kyoung; Yoshifumi Nishikawa; Naoaki Yokoyama; Xuenan Xuan; Ikuo Igarashi

doi:10.1016/j.parint.2012.02.006

C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses

Mohamad Alaa Terkawi, Hend Alhasan, Akio Ueno, Jadsada Ratthanophart, Yuzi Luo, Shinuo Cao, Ketsarin Kamyingkird, Mahmoud Aboulaila, Goo Youn-Kyoung, Yoshifumi Nishikawa, Naoaki Yokoyama, Xuenan Xuan, Ikuo Igarashi

Department of Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A recombinant C-terminal antigen derived from Babesia caballi 48-kDa rhoptry protein (rBc48/CT) was made for the development of a serologically diagnostic test. Antiserum raised against the rBc48/CT reacted specifically with the corresponding native protein by Western blotting and the indirect fluorescent antibody test (IFAT). Next, an indirect enzyme-linked immunosorbent assay (Bc48/CT-ELISA) and an immunochromatographic test based on the Bc48/CT (Bc48/CT-ICT) were constructed and employed for the detection of an antibody to B. caballi in a variety of equine sera. The results of Bc48/CT-ELISA and Bc48/CT-ICT were highly concordant with those of IFAT and ELISA, with full-length protein of Bc48 used as the reference tests. Our results demonstrate the success of Bc48/CT as antigen for the serological diagnosis of B. caballi infection in horses.

Original language	English
Pages (from-to)	493-496
Number of pages	4
Journal	Parasitology International
Volume	61
Issue number	3
DOIs	https://doi.org/10.1016/j.parint.2012.02.006
State	Published - Sep 2012

Keywords

48-kDa Rhoptry protein
Babesia caballi
Diagnosis
ELISA
Immunochromatographic test

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.parint.2012.02.006

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Terkawi, M. A., Alhasan, H., Ueno, A., Ratthanophart, J., Luo, Y., Cao, S., Kamyingkird, K., Aboulaila, M., Youn-Kyoung, G., Nishikawa, Y., Yokoyama, N., Xuan, X., & Igarashi, I. (2012). C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses. Parasitology International, 61(3), 493-496. https://doi.org/10.1016/j.parint.2012.02.006

@article{7337b85213ac45cf91d31f1377d9c443,

title = "C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses",

abstract = "A recombinant C-terminal antigen derived from Babesia caballi 48-kDa rhoptry protein (rBc48/CT) was made for the development of a serologically diagnostic test. Antiserum raised against the rBc48/CT reacted specifically with the corresponding native protein by Western blotting and the indirect fluorescent antibody test (IFAT). Next, an indirect enzyme-linked immunosorbent assay (Bc48/CT-ELISA) and an immunochromatographic test based on the Bc48/CT (Bc48/CT-ICT) were constructed and employed for the detection of an antibody to B. caballi in a variety of equine sera. The results of Bc48/CT-ELISA and Bc48/CT-ICT were highly concordant with those of IFAT and ELISA, with full-length protein of Bc48 used as the reference tests. Our results demonstrate the success of Bc48/CT as antigen for the serological diagnosis of B. caballi infection in horses.",

keywords = "48-kDa Rhoptry protein, Babesia caballi, Diagnosis, ELISA, Immunochromatographic test",

author = "Terkawi, {Mohamad Alaa} and Hend Alhasan and Akio Ueno and Jadsada Ratthanophart and Yuzi Luo and Shinuo Cao and Ketsarin Kamyingkird and Mahmoud Aboulaila and Goo Youn-Kyoung and Yoshifumi Nishikawa and Naoaki Yokoyama and Xuenan Xuan and Ikuo Igarashi",

year = "2012",

month = sep,

doi = "10.1016/j.parint.2012.02.006",

language = "English",

volume = "61",

pages = "493--496",

journal = "Parasitology International",

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Terkawi, MA, Alhasan, H, Ueno, A, Ratthanophart, J, Luo, Y, Cao, S, Kamyingkird, K, Aboulaila, M, Youn-Kyoung, G, Nishikawa, Y, Yokoyama, N, Xuan, X & Igarashi, I 2012, 'C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses', Parasitology International, vol. 61, no. 3, pp. 493-496. https://doi.org/10.1016/j.parint.2012.02.006

TY - JOUR

T1 - C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses

AU - Terkawi, Mohamad Alaa

AU - Alhasan, Hend

AU - Ueno, Akio

AU - Ratthanophart, Jadsada

AU - Luo, Yuzi

AU - Cao, Shinuo

AU - Kamyingkird, Ketsarin

AU - Aboulaila, Mahmoud

AU - Youn-Kyoung, Goo

AU - Nishikawa, Yoshifumi

AU - Yokoyama, Naoaki

AU - Xuan, Xuenan

AU - Igarashi, Ikuo

PY - 2012/9

Y1 - 2012/9

N2 - A recombinant C-terminal antigen derived from Babesia caballi 48-kDa rhoptry protein (rBc48/CT) was made for the development of a serologically diagnostic test. Antiserum raised against the rBc48/CT reacted specifically with the corresponding native protein by Western blotting and the indirect fluorescent antibody test (IFAT). Next, an indirect enzyme-linked immunosorbent assay (Bc48/CT-ELISA) and an immunochromatographic test based on the Bc48/CT (Bc48/CT-ICT) were constructed and employed for the detection of an antibody to B. caballi in a variety of equine sera. The results of Bc48/CT-ELISA and Bc48/CT-ICT were highly concordant with those of IFAT and ELISA, with full-length protein of Bc48 used as the reference tests. Our results demonstrate the success of Bc48/CT as antigen for the serological diagnosis of B. caballi infection in horses.

AB - A recombinant C-terminal antigen derived from Babesia caballi 48-kDa rhoptry protein (rBc48/CT) was made for the development of a serologically diagnostic test. Antiserum raised against the rBc48/CT reacted specifically with the corresponding native protein by Western blotting and the indirect fluorescent antibody test (IFAT). Next, an indirect enzyme-linked immunosorbent assay (Bc48/CT-ELISA) and an immunochromatographic test based on the Bc48/CT (Bc48/CT-ICT) were constructed and employed for the detection of an antibody to B. caballi in a variety of equine sera. The results of Bc48/CT-ELISA and Bc48/CT-ICT were highly concordant with those of IFAT and ELISA, with full-length protein of Bc48 used as the reference tests. Our results demonstrate the success of Bc48/CT as antigen for the serological diagnosis of B. caballi infection in horses.

KW - 48-kDa Rhoptry protein

KW - Babesia caballi

KW - Diagnosis

KW - ELISA

KW - Immunochromatographic test

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M3 - Article

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AN - SCOPUS:84861482934

SN - 1383-5769

VL - 61

SP - 493

EP - 496

JO - Parasitology International

JF - Parasitology International

IS - 3

ER -

C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses

Abstract

Keywords

UN SDGs

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