C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses

Mohamad Alaa Terkawi; Hend Alhasan; Akio Ueno; Jadsada Ratthanophart; Yuzi Luo; Shinuo Cao; Ketsarin Kamyingkird; Mahmoud Aboulaila; Goo Youn-Kyoung; Yoshifumi Nishikawa; Naoaki Yokoyama; Xuenan Xuan; Ikuo Igarashi

doi:10.1016/j.parint.2012.02.006

C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses

Mohamad Alaa Terkawi
, Hend Alhasan
, Akio Ueno
, Jadsada Ratthanophart
, Yuzi Luo
, Shinuo Cao
, Ketsarin Kamyingkird
, Mahmoud Aboulaila
, Goo Youn-Kyoung
, Yoshifumi Nishikawa
, Naoaki Yokoyama
, Xuenan Xuan
, Ikuo Igarashi

Department of Medicine

Obihiro University of Agriculture and Veterinary Medicine
Menoufia University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A recombinant C-terminal antigen derived from Babesia caballi 48-kDa rhoptry protein (rBc48/CT) was made for the development of a serologically diagnostic test. Antiserum raised against the rBc48/CT reacted specifically with the corresponding native protein by Western blotting and the indirect fluorescent antibody test (IFAT). Next, an indirect enzyme-linked immunosorbent assay (Bc48/CT-ELISA) and an immunochromatographic test based on the Bc48/CT (Bc48/CT-ICT) were constructed and employed for the detection of an antibody to B. caballi in a variety of equine sera. The results of Bc48/CT-ELISA and Bc48/CT-ICT were highly concordant with those of IFAT and ELISA, with full-length protein of Bc48 used as the reference tests. Our results demonstrate the success of Bc48/CT as antigen for the serological diagnosis of B. caballi infection in horses.

Original language	English
Pages (from-to)	493-496
Number of pages	4
Journal	Parasitology International
Volume	61
Issue number	3
DOIs	https://doi.org/10.1016/j.parint.2012.02.006
State	Published - Sep 2012

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

48-kDa Rhoptry protein
Babesia caballi
Diagnosis
ELISA
Immunochromatographic test

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10.1016/j.parint.2012.02.006

Cite this

Terkawi, M. A., Alhasan, H., Ueno, A., Ratthanophart, J., Luo, Y., Cao, S., Kamyingkird, K., Aboulaila, M., Youn-Kyoung, G., Nishikawa, Y., Yokoyama, N., Xuan, X., & Igarashi, I. (2012). C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses. Parasitology International, 61(3), 493-496. https://doi.org/10.1016/j.parint.2012.02.006

@article{7337b85213ac45cf91d31f1377d9c443,

title = "C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses",

abstract = "A recombinant C-terminal antigen derived from Babesia caballi 48-kDa rhoptry protein (rBc48/CT) was made for the development of a serologically diagnostic test. Antiserum raised against the rBc48/CT reacted specifically with the corresponding native protein by Western blotting and the indirect fluorescent antibody test (IFAT). Next, an indirect enzyme-linked immunosorbent assay (Bc48/CT-ELISA) and an immunochromatographic test based on the Bc48/CT (Bc48/CT-ICT) were constructed and employed for the detection of an antibody to B. caballi in a variety of equine sera. The results of Bc48/CT-ELISA and Bc48/CT-ICT were highly concordant with those of IFAT and ELISA, with full-length protein of Bc48 used as the reference tests. Our results demonstrate the success of Bc48/CT as antigen for the serological diagnosis of B. caballi infection in horses.",

keywords = "48-kDa Rhoptry protein, Babesia caballi, Diagnosis, ELISA, Immunochromatographic test",

author = "Terkawi, \{Mohamad Alaa\} and Hend Alhasan and Akio Ueno and Jadsada Ratthanophart and Yuzi Luo and Shinuo Cao and Ketsarin Kamyingkird and Mahmoud Aboulaila and Goo Youn-Kyoung and Yoshifumi Nishikawa and Naoaki Yokoyama and Xuenan Xuan and Ikuo Igarashi",

year = "2012",

month = sep,

doi = "10.1016/j.parint.2012.02.006",

language = "English",

volume = "61",

pages = "493--496",

journal = "Parasitology International",

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Terkawi, MA, Alhasan, H, Ueno, A, Ratthanophart, J, Luo, Y, Cao, S, Kamyingkird, K, Aboulaila, M, Youn-Kyoung, G, Nishikawa, Y, Yokoyama, N, Xuan, X & Igarashi, I 2012, 'C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses', Parasitology International, vol. 61, no. 3, pp. 493-496. https://doi.org/10.1016/j.parint.2012.02.006

TY - JOUR

T1 - C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses

AU - Terkawi, Mohamad Alaa

AU - Alhasan, Hend

AU - Ueno, Akio

AU - Ratthanophart, Jadsada

AU - Luo, Yuzi

AU - Cao, Shinuo

AU - Kamyingkird, Ketsarin

AU - Aboulaila, Mahmoud

AU - Youn-Kyoung, Goo

AU - Nishikawa, Yoshifumi

AU - Yokoyama, Naoaki

AU - Xuan, Xuenan

AU - Igarashi, Ikuo

PY - 2012/9

Y1 - 2012/9

N2 - A recombinant C-terminal antigen derived from Babesia caballi 48-kDa rhoptry protein (rBc48/CT) was made for the development of a serologically diagnostic test. Antiserum raised against the rBc48/CT reacted specifically with the corresponding native protein by Western blotting and the indirect fluorescent antibody test (IFAT). Next, an indirect enzyme-linked immunosorbent assay (Bc48/CT-ELISA) and an immunochromatographic test based on the Bc48/CT (Bc48/CT-ICT) were constructed and employed for the detection of an antibody to B. caballi in a variety of equine sera. The results of Bc48/CT-ELISA and Bc48/CT-ICT were highly concordant with those of IFAT and ELISA, with full-length protein of Bc48 used as the reference tests. Our results demonstrate the success of Bc48/CT as antigen for the serological diagnosis of B. caballi infection in horses.

AB - A recombinant C-terminal antigen derived from Babesia caballi 48-kDa rhoptry protein (rBc48/CT) was made for the development of a serologically diagnostic test. Antiserum raised against the rBc48/CT reacted specifically with the corresponding native protein by Western blotting and the indirect fluorescent antibody test (IFAT). Next, an indirect enzyme-linked immunosorbent assay (Bc48/CT-ELISA) and an immunochromatographic test based on the Bc48/CT (Bc48/CT-ICT) were constructed and employed for the detection of an antibody to B. caballi in a variety of equine sera. The results of Bc48/CT-ELISA and Bc48/CT-ICT were highly concordant with those of IFAT and ELISA, with full-length protein of Bc48 used as the reference tests. Our results demonstrate the success of Bc48/CT as antigen for the serological diagnosis of B. caballi infection in horses.

KW - 48-kDa Rhoptry protein

KW - Babesia caballi

KW - Diagnosis

KW - ELISA

KW - Immunochromatographic test

UR - https://www.scopus.com/pages/publications/84861482934

U2 - 10.1016/j.parint.2012.02.006

DO - 10.1016/j.parint.2012.02.006

M3 - Article

C2 - 22387861

AN - SCOPUS:84861482934

SN - 1383-5769

VL - 61

SP - 493

EP - 496

JO - Parasitology International

JF - Parasitology International

IS - 3

ER -

C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses

Abstract

UN SDGs

Keywords

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