Cadophora malorum Cs-8-1 as a new fungal strain producing gibberellins isolated from Calystegia soldanella

Fourteen endophytic fungi with different colony morphologies were isolated from the roots of Calystegia soldanella. Endophytic fungi isolated from C. soldanella were identified by internal transcribed spacer (ITS) region. To verify plant growth promotion (PGP), culture filtrates of isolated endophytic fungi were treated in Waito-c rice (WR) and C. soldanella seedlings. Culture filtrates of Cs-8-1 fungal strain had advanced PGP activity. The presence of physiologically bioactive gibberellins (GA) GA₁ (1.213ngml^-1), GA₃ (1.292ngml^-1), GA₄ (3.6ngml^-1), GA₇ (1.328ngml^-1), other inactive GA₉ (0.796ngml^-1) and GA₁₂ (0.417ngml^-1), GA₂₀ (0.302ngml^-1), GA₂₄ (1.351ngml^-1), GA₃₄ (0.076ngml^-1), and GA₅₃ (0.051ngml^-1) in culture filtrates of Cs-8-1 fungal strain was detected. The Cs-8-1 fungal strain was confirmed as a producer of GAs. Molecular analysis of sequences showed high similarity of 99% to Cadophora malorum. Consequentially, the Cs-8-1 fungal strain was identified as a new C. malorum producing GAs.