TY - JOUR
T1 - Capillary gel electrophoretic analysis of cattle breeds based on difference of DNA mobility of microsatellite markers
AU - Lee, Miji
AU - Yoon, Duhak
AU - Jeon, Jin Tae
AU - Eo, Seong Kug
AU - Kang, Seong Ho
PY - 2009/11
Y1 - 2009/11
N2 - A breed of cattle, i.e., Korean cattle (Hanwoo), was identified based on the DNA mobilities of their microsatellites (MSs) by capillary gel electrophoresis (CGE) with a laser-induced fluorescence (LIF) detector. The MS markers were used for the accurate identification of species-specific genes. The DNA mobilities of the MS markers of Hanwoo and Holstein were measured using a CGE system with a fused-silica capillary (inner diameter of 75 μm, outer diameter of 365 μm, and total length of 50 cm). The capillary was dynamically coated with 1.0% (w/v) polyvinylpyrrolidone (Mr = 1,000,000) and then filled with a mixture of 1.3% (w/v) poly(ethylene oxide) (Mr = 600,000) and 1.9% (w/v) poly(ethylene oxide) (Mr = 8,000,000) as a sieving gel matrix. The species-specific genes of Hanwoo and Holstein were clearly distinguished within 33 min. This CGE assay technique is expected to be a useful analytical method for the fast and accurate identification of breeds of cattle.
AB - A breed of cattle, i.e., Korean cattle (Hanwoo), was identified based on the DNA mobilities of their microsatellites (MSs) by capillary gel electrophoresis (CGE) with a laser-induced fluorescence (LIF) detector. The MS markers were used for the accurate identification of species-specific genes. The DNA mobilities of the MS markers of Hanwoo and Holstein were measured using a CGE system with a fused-silica capillary (inner diameter of 75 μm, outer diameter of 365 μm, and total length of 50 cm). The capillary was dynamically coated with 1.0% (w/v) polyvinylpyrrolidone (Mr = 1,000,000) and then filled with a mixture of 1.3% (w/v) poly(ethylene oxide) (Mr = 600,000) and 1.9% (w/v) poly(ethylene oxide) (Mr = 8,000,000) as a sieving gel matrix. The species-specific genes of Hanwoo and Holstein were clearly distinguished within 33 min. This CGE assay technique is expected to be a useful analytical method for the fast and accurate identification of breeds of cattle.
KW - Capillary gel electrophoresis
KW - Cattle breed
KW - DNA mobility
KW - Microsatellite
UR - http://www.scopus.com/inward/record.url?scp=75849162451&partnerID=8YFLogxK
U2 - 10.5012/bkcs.2009.30.11.2655
DO - 10.5012/bkcs.2009.30.11.2655
M3 - Article
AN - SCOPUS:75849162451
SN - 0253-2964
VL - 30
SP - 2655
EP - 2660
JO - Bulletin of the Korean Chemical Society
JF - Bulletin of the Korean Chemical Society
IS - 11
ER -