Cationic cellulose nanocrystals complexed with polymeric siRNA for efficient anticancer drug delivery

Young Min Kim; Yoon Suk Lee; Taehyung Kim; Kyungjik Yang; Keonwook Nam; Deokyeong Choe; Young Hoon Roh

doi:10.1016/j.carbpol.2020.116684

Cationic cellulose nanocrystals complexed with polymeric siRNA for efficient anticancer drug delivery

Young Min Kim, Yoon Suk Lee, Taehyung Kim, Kyungjik Yang, Keonwook Nam, Deokyeong Choe, Young Hoon Roh

School of Food Science & Biotechnology

Yonsei University

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Surface-modified cellulose nanocrystals (CNCs) were developed for efficient delivery of polymeric siRNA in cancer cells. Cationic CNCs were synthesized using the sequential process of hydrothermal desulfation and chemical modification following which, polymeric siRNA obtained using from a two-step process of rolling circle transcription and Mg²⁺ chelation was complexed with the modified CNCs by electrostatic interaction. The complexation efficiency was optimized for high drug loading and release in the cytoplasmic environment. The resultant nanocomplex showed significantly enhanced enzymatic stability, gene knockdown efficacy, and apoptosis-induced in vitro therapeutic effect. Our results suggest CNCs as a promising carbohydrate-based delivery platform which could be utilized for RNAi-mediated cancer therapeutics.

Original language	English
Article number	116684
Journal	Carbohydrate Polymers
Volume	247
DOIs	https://doi.org/10.1016/j.carbpol.2020.116684
State	Published - 1 Nov 2020

Keywords

Cellulose nanocrystals
Hydrothermal desulfation
Nanocomplex
Rolling circle transcription
siRNA delivery

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.carbpol.2020.116684

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title = "Cationic cellulose nanocrystals complexed with polymeric siRNA for efficient anticancer drug delivery",

abstract = "Surface-modified cellulose nanocrystals (CNCs) were developed for efficient delivery of polymeric siRNA in cancer cells. Cationic CNCs were synthesized using the sequential process of hydrothermal desulfation and chemical modification following which, polymeric siRNA obtained using from a two-step process of rolling circle transcription and Mg2+ chelation was complexed with the modified CNCs by electrostatic interaction. The complexation efficiency was optimized for high drug loading and release in the cytoplasmic environment. The resultant nanocomplex showed significantly enhanced enzymatic stability, gene knockdown efficacy, and apoptosis-induced in vitro therapeutic effect. Our results suggest CNCs as a promising carbohydrate-based delivery platform which could be utilized for RNAi-mediated cancer therapeutics.",

keywords = "Cellulose nanocrystals, Hydrothermal desulfation, Nanocomplex, Rolling circle transcription, siRNA delivery",

author = "Kim, {Young Min} and Lee, {Yoon Suk} and Taehyung Kim and Kyungjik Yang and Keonwook Nam and Deokyeong Choe and Roh, {Young Hoon}",

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doi = "10.1016/j.carbpol.2020.116684",

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T1 - Cationic cellulose nanocrystals complexed with polymeric siRNA for efficient anticancer drug delivery

AU - Kim, Young Min

AU - Lee, Yoon Suk

AU - Kim, Taehyung

AU - Yang, Kyungjik

AU - Nam, Keonwook

AU - Choe, Deokyeong

AU - Roh, Young Hoon

PY - 2020/11/1

Y1 - 2020/11/1

N2 - Surface-modified cellulose nanocrystals (CNCs) were developed for efficient delivery of polymeric siRNA in cancer cells. Cationic CNCs were synthesized using the sequential process of hydrothermal desulfation and chemical modification following which, polymeric siRNA obtained using from a two-step process of rolling circle transcription and Mg2+ chelation was complexed with the modified CNCs by electrostatic interaction. The complexation efficiency was optimized for high drug loading and release in the cytoplasmic environment. The resultant nanocomplex showed significantly enhanced enzymatic stability, gene knockdown efficacy, and apoptosis-induced in vitro therapeutic effect. Our results suggest CNCs as a promising carbohydrate-based delivery platform which could be utilized for RNAi-mediated cancer therapeutics.

AB - Surface-modified cellulose nanocrystals (CNCs) were developed for efficient delivery of polymeric siRNA in cancer cells. Cationic CNCs were synthesized using the sequential process of hydrothermal desulfation and chemical modification following which, polymeric siRNA obtained using from a two-step process of rolling circle transcription and Mg2+ chelation was complexed with the modified CNCs by electrostatic interaction. The complexation efficiency was optimized for high drug loading and release in the cytoplasmic environment. The resultant nanocomplex showed significantly enhanced enzymatic stability, gene knockdown efficacy, and apoptosis-induced in vitro therapeutic effect. Our results suggest CNCs as a promising carbohydrate-based delivery platform which could be utilized for RNAi-mediated cancer therapeutics.

KW - Cellulose nanocrystals

KW - Hydrothermal desulfation

KW - Nanocomplex

KW - Rolling circle transcription

KW - siRNA delivery

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DO - 10.1016/j.carbpol.2020.116684

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JO - Carbohydrate Polymers

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Cationic cellulose nanocrystals complexed with polymeric siRNA for efficient anticancer drug delivery

Abstract

Keywords

UN SDGs

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