CDNA cloning and analysis of tissue-specific gene expression of rat urocortin II

J. I. Chae; S. K. Ju; M. K. Lee; J. H. Park; J. H. Yoon; J. H. Shim; D. S. Lee

doi:10.1134/S0026893309010117

CDNA cloning and analysis of tissue-specific gene expression of rat urocortin II

J. I. Chae, S. K. Ju, M. K. Lee, J. H. Park, J. H. Yoon, J. H. Shim, D. S. Lee

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1 Scopus citations

Abstract

The corticotropin-releasing hormone (CRH) family of neuropeptides includes CRH (a 41-amino acid hypothalamic peptide) and urocortin. Corticotropin- releasing factor (CRF), a peptide first isolated from mammalian, plays an important role in the regulation of the pituitary-adrenal axis, and in endocrine, autonomic, immune and behavioral responses to stress. In this study, we cloned rat urocortin II (UCN II) cDNA from rat mid-brain by RT-PCR. The rat UCN II clone contained an open reading frame (ORF) 109 amino acids which shared 90% and 63% homology with mouse and human homologues, respectively. The expression of UCN II mRNA is mainly distributed in bone marrow, ovary, uterus, hypophysis, adrenal gland, and skin. In this study, rat recombinant UCN was expressed in E. coli and purified in active form. Furthermore, purified recombinant UCN II protein specifically binds to CRF receptor 2 in rat ROS 17 / 2.8 and GH3 cells by flow-cytometry analysis. UCN II cDNA clone obtained in this study will be useful for further investigation on behavioral responses to stress in rats.

Original language	English
Pages (from-to)	82-87
Number of pages	6
Journal	Molecular Biology
Volume	43
Issue number	1
DOIs	https://doi.org/10.1134/S0026893309010117
State	Published - Feb 2009

Keywords

FACS
Recombinant proteins
Urocortin II

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10.1134/S0026893309010117

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title = "CDNA cloning and analysis of tissue-specific gene expression of rat urocortin II",

abstract = "The corticotropin-releasing hormone (CRH) family of neuropeptides includes CRH (a 41-amino acid hypothalamic peptide) and urocortin. Corticotropin- releasing factor (CRF), a peptide first isolated from mammalian, plays an important role in the regulation of the pituitary-adrenal axis, and in endocrine, autonomic, immune and behavioral responses to stress. In this study, we cloned rat urocortin II (UCN II) cDNA from rat mid-brain by RT-PCR. The rat UCN II clone contained an open reading frame (ORF) 109 amino acids which shared 90% and 63% homology with mouse and human homologues, respectively. The expression of UCN II mRNA is mainly distributed in bone marrow, ovary, uterus, hypophysis, adrenal gland, and skin. In this study, rat recombinant UCN was expressed in E. coli and purified in active form. Furthermore, purified recombinant UCN II protein specifically binds to CRF receptor 2 in rat ROS 17 / 2.8 and GH3 cells by flow-cytometry analysis. UCN II cDNA clone obtained in this study will be useful for further investigation on behavioral responses to stress in rats.",

keywords = "FACS, Recombinant proteins, Urocortin II",

author = "Chae, {J. I.} and Ju, {S. K.} and Lee, {M. K.} and Park, {J. H.} and Yoon, {J. H.} and Shim, {J. H.} and Lee, {D. S.}",

year = "2009",

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T1 - CDNA cloning and analysis of tissue-specific gene expression of rat urocortin II

AU - Chae, J. I.

AU - Ju, S. K.

AU - Lee, M. K.

AU - Park, J. H.

AU - Yoon, J. H.

AU - Shim, J. H.

AU - Lee, D. S.

PY - 2009/2

Y1 - 2009/2

N2 - The corticotropin-releasing hormone (CRH) family of neuropeptides includes CRH (a 41-amino acid hypothalamic peptide) and urocortin. Corticotropin- releasing factor (CRF), a peptide first isolated from mammalian, plays an important role in the regulation of the pituitary-adrenal axis, and in endocrine, autonomic, immune and behavioral responses to stress. In this study, we cloned rat urocortin II (UCN II) cDNA from rat mid-brain by RT-PCR. The rat UCN II clone contained an open reading frame (ORF) 109 amino acids which shared 90% and 63% homology with mouse and human homologues, respectively. The expression of UCN II mRNA is mainly distributed in bone marrow, ovary, uterus, hypophysis, adrenal gland, and skin. In this study, rat recombinant UCN was expressed in E. coli and purified in active form. Furthermore, purified recombinant UCN II protein specifically binds to CRF receptor 2 in rat ROS 17 / 2.8 and GH3 cells by flow-cytometry analysis. UCN II cDNA clone obtained in this study will be useful for further investigation on behavioral responses to stress in rats.

AB - The corticotropin-releasing hormone (CRH) family of neuropeptides includes CRH (a 41-amino acid hypothalamic peptide) and urocortin. Corticotropin- releasing factor (CRF), a peptide first isolated from mammalian, plays an important role in the regulation of the pituitary-adrenal axis, and in endocrine, autonomic, immune and behavioral responses to stress. In this study, we cloned rat urocortin II (UCN II) cDNA from rat mid-brain by RT-PCR. The rat UCN II clone contained an open reading frame (ORF) 109 amino acids which shared 90% and 63% homology with mouse and human homologues, respectively. The expression of UCN II mRNA is mainly distributed in bone marrow, ovary, uterus, hypophysis, adrenal gland, and skin. In this study, rat recombinant UCN was expressed in E. coli and purified in active form. Furthermore, purified recombinant UCN II protein specifically binds to CRF receptor 2 in rat ROS 17 / 2.8 and GH3 cells by flow-cytometry analysis. UCN II cDNA clone obtained in this study will be useful for further investigation on behavioral responses to stress in rats.

KW - FACS

KW - Recombinant proteins

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DO - 10.1134/S0026893309010117

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JF - Molecular Biology

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ER -

CDNA cloning and analysis of tissue-specific gene expression of rat urocortin II

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Keywords

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