cDNA cloning and analysis of tissue-specific gene expression of rat urocortin II

J. I. Chae; S. K. Ju; M. K. Lee; J. H. Park; J. H. Yoon; J. H. Shim; D. S. Lee

cDNA cloning and analysis of tissue-specific gene expression of rat urocortin II

J. I. Chae
, S. K. Ju
, M. K. Lee
, J. H. Park
, J. H. Yoon
, J. H. Shim
, D. S. Lee

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The corticotropin-releasing hormone (CRH) family of neuropeptides includes CRH (a 41 amino acid hypothalamic peptide) and urocortin. Corticotropin-releasing factor (CRF), a peptide first isolated from mammalian, plays an important role in the regulation of the pituitary-adrenal axis, and in endocrine, autonomic, immune and behavioral responses to stress. In this study we cloned rat urocortin II (UCNII) cDNA from rat mid-brain by RT-PCR. The rat UCNII clone contained an open reading frame (ORF) coding 109 amino acids which shared 90% and 63% homology with mouse and human homologues, respectively, The expression of UCN HII mRNA is mainly distributed in bone marrow, ovary, uterus, hypophysis, adrenal gland, and skin. In this study, rat recombinant UCN was expressed in E. coli and purified in active form. Furthermore, purified recombinant UCN II protein specifically binds to CRF receptor 2 in rat ROS 17/2.8 and GH3 cells by flow-cytometry analysis. UCN II cDNA clone obtained in this study will be useful for further investigation on behavioral responses to stress in rats.

Original language	English
Pages (from-to)	91-96
Number of pages	6
Journal	Molekulyarnaya Biologiya
Volume	43
Issue number	1
State	Published - Jan 2009

Cite this

@article{9d0e49b101b34f6cbe0962131d8042f4,

title = "cDNA cloning and analysis of tissue-specific gene expression of rat urocortin II",

abstract = "The corticotropin-releasing hormone (CRH) family of neuropeptides includes CRH (a 41 amino acid hypothalamic peptide) and urocortin. Corticotropin-releasing factor (CRF), a peptide first isolated from mammalian, plays an important role in the regulation of the pituitary-adrenal axis, and in endocrine, autonomic, immune and behavioral responses to stress. In this study we cloned rat urocortin II (UCNII) cDNA from rat mid-brain by RT-PCR. The rat UCNII clone contained an open reading frame (ORF) coding 109 amino acids which shared 90\% and 63\% homology with mouse and human homologues, respectively, The expression of UCN HII mRNA is mainly distributed in bone marrow, ovary, uterus, hypophysis, adrenal gland, and skin. In this study, rat recombinant UCN was expressed in E. coli and purified in active form. Furthermore, purified recombinant UCN II protein specifically binds to CRF receptor 2 in rat ROS 17/2.8 and GH3 cells by flow-cytometry analysis. UCN II cDNA clone obtained in this study will be useful for further investigation on behavioral responses to stress in rats.",

author = "Chae, \{J. I.\} and Ju, \{S. K.\} and Lee, \{M. K.\} and Park, \{J. H.\} and Yoon, \{J. H.\} and Shim, \{J. H.\} and Lee, \{D. S.\}",

year = "2009",

month = jan,

language = "English",

volume = "43",

pages = "91--96",

journal = "Molekulyarnaya Biologiya",

issn = "0026-8984",

publisher = "Russian Academy of Sciences",

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}

TY - JOUR

T1 - cDNA cloning and analysis of tissue-specific gene expression of rat urocortin II

AU - Chae, J. I.

AU - Ju, S. K.

AU - Lee, M. K.

AU - Park, J. H.

AU - Yoon, J. H.

AU - Shim, J. H.

AU - Lee, D. S.

PY - 2009/1

Y1 - 2009/1

N2 - The corticotropin-releasing hormone (CRH) family of neuropeptides includes CRH (a 41 amino acid hypothalamic peptide) and urocortin. Corticotropin-releasing factor (CRF), a peptide first isolated from mammalian, plays an important role in the regulation of the pituitary-adrenal axis, and in endocrine, autonomic, immune and behavioral responses to stress. In this study we cloned rat urocortin II (UCNII) cDNA from rat mid-brain by RT-PCR. The rat UCNII clone contained an open reading frame (ORF) coding 109 amino acids which shared 90% and 63% homology with mouse and human homologues, respectively, The expression of UCN HII mRNA is mainly distributed in bone marrow, ovary, uterus, hypophysis, adrenal gland, and skin. In this study, rat recombinant UCN was expressed in E. coli and purified in active form. Furthermore, purified recombinant UCN II protein specifically binds to CRF receptor 2 in rat ROS 17/2.8 and GH3 cells by flow-cytometry analysis. UCN II cDNA clone obtained in this study will be useful for further investigation on behavioral responses to stress in rats.

AB - The corticotropin-releasing hormone (CRH) family of neuropeptides includes CRH (a 41 amino acid hypothalamic peptide) and urocortin. Corticotropin-releasing factor (CRF), a peptide first isolated from mammalian, plays an important role in the regulation of the pituitary-adrenal axis, and in endocrine, autonomic, immune and behavioral responses to stress. In this study we cloned rat urocortin II (UCNII) cDNA from rat mid-brain by RT-PCR. The rat UCNII clone contained an open reading frame (ORF) coding 109 amino acids which shared 90% and 63% homology with mouse and human homologues, respectively, The expression of UCN HII mRNA is mainly distributed in bone marrow, ovary, uterus, hypophysis, adrenal gland, and skin. In this study, rat recombinant UCN was expressed in E. coli and purified in active form. Furthermore, purified recombinant UCN II protein specifically binds to CRF receptor 2 in rat ROS 17/2.8 and GH3 cells by flow-cytometry analysis. UCN II cDNA clone obtained in this study will be useful for further investigation on behavioral responses to stress in rats.

UR - https://www.scopus.com/pages/publications/65349159738

M3 - Article

C2 - 19334530

AN - SCOPUS:65349159738

SN - 0026-8984

VL - 43

SP - 91

EP - 96

JO - Molekulyarnaya Biologiya

JF - Molekulyarnaya Biologiya

IS - 1

ER -

cDNA cloning and analysis of tissue-specific gene expression of rat urocortin II

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