cDNA cloning and expression of ATP synthase subunit b from the fire-bellied frog Bombina orientalis (Anura: Discoglossidae)

Min Sun Kim; Il Chan Kim; Sang Soo Kim; Sook Kyoung Kim; Young Mi Lee; Han Seung Kang; Hee Seok Kweon; Ui Wook Hwang; Moon Kyoo Kim; Jae Seong Lee; Yong Dal Yoon

doi:10.1080/10425170410001723930

cDNA cloning and expression of ATP synthase subunit b from the fire-bellied frog Bombina orientalis (Anura: Discoglossidae)

Min Sun Kim, Il Chan Kim, Sang Soo Kim, Sook Kyoung Kim, Young Mi Lee, Han Seung Kang, Hee Seok Kweon, Ui Wook Hwang, Moon Kyoo Kim, Jae Seong Lee, Yong Dal Yoon

Department of Biology Education

Hanyang University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We cloned the Bombina orientalis adenosine triphosphate (ATP) synthase subunit b gene from a B. orientalis oviduct cDNA library. The transcript was 997 bp long and encoded 250 amino acid residues. It showed high similarity to amphibian (84-85%), mammalian (56-62%) and invertebrate (46-50%) sequences. In phylogenetic analyses, it clustered with other amphibian sequences. This gene was highly expressed in brain, intestine and oviduct but not in muscle and liver. In this paper, we report the basic characteristics of B. orientalis ATP synthase subunit b gene.

Original language	English
Pages (from-to)	196-201
Number of pages	6
Journal	DNA Sequence - Journal of DNA Sequencing and Mapping
Volume	15
Issue number	3
DOIs	https://doi.org/10.1080/10425170410001723930
State	Published - Jun 2004

Keywords

ATP synthase
Bombina orientalis
CDNA
Frog

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10.1080/10425170410001723930

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Kim, M. S., Kim, I. C., Kim, S. S., Kim, S. K., Lee, Y. M., Kang, H. S., Kweon, H. S., Hwang, U. W., Kim, M. K., Lee, J. S., & Yoon, Y. D. (2004). cDNA cloning and expression of ATP synthase subunit b from the fire-bellied frog Bombina orientalis (Anura: Discoglossidae). DNA Sequence - Journal of DNA Sequencing and Mapping, 15(3), 196-201. https://doi.org/10.1080/10425170410001723930

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abstract = "We cloned the Bombina orientalis adenosine triphosphate (ATP) synthase subunit b gene from a B. orientalis oviduct cDNA library. The transcript was 997 bp long and encoded 250 amino acid residues. It showed high similarity to amphibian (84-85%), mammalian (56-62%) and invertebrate (46-50%) sequences. In phylogenetic analyses, it clustered with other amphibian sequences. This gene was highly expressed in brain, intestine and oviduct but not in muscle and liver. In this paper, we report the basic characteristics of B. orientalis ATP synthase subunit b gene.",

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AU - Kim, Il Chan

AU - Kim, Sang Soo

AU - Kim, Sook Kyoung

AU - Lee, Young Mi

AU - Kang, Han Seung

AU - Kweon, Hee Seok

AU - Hwang, Ui Wook

AU - Kim, Moon Kyoo

AU - Lee, Jae Seong

AU - Yoon, Yong Dal

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cDNA cloning and expression of ATP synthase subunit b from the fire-bellied frog Bombina orientalis (Anura: Discoglossidae)

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Keywords

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