Cellulomonas fulva sp. nov., isolated from oil-contaminated soil

A yellow-coloured, Gram-stain-positive, motile, aerobic and rod-shaped bacteria, designated DKR-3^T, was isolated from oil-contaminated experimental soil. Strain DKR-3^T could grow at pH 5.0–10.5 (optimum, pH 7.0–8.5), at 10–40 °C (optimum, 25–32 °C) and tolerated 3.5% of NaCl. Phylogenetic analyses based on its 16S rRNA gene sequence indicated that strain DKR3^T formed a lineage within the family Cellulomonadaceae and was clustered with members of the genus Cellulomonas. Strain DKR-3^T had highest 16S rRNA gene sequence similarities to Cellulomonas gelida DSM 20111^T (98.3%), Cellulomonas persica JCM 18111^T (98.2%) and Cellulomonas uda DSM 20107^T (97.8%). The predominant respiratory quinone was tetrahydrogenated menaquinone with nine isoprene units [MK-9(H₄)]. The principal cellular fatty acids were anteiso-C_15:0, C_16:0 and anteiso-C_17:0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The cell-wall diamino acid was l-ornithine whereas rhamnose and glucose were the cell-wall sugars. The DNA G+C content was 74.2mol%. The genome of strain DKR-3^T was 3.74 Mb and contained three putative biosynthetic gene clusters. The average nucleotide identity and digital DNA–DNA hybridization relatedness values between strain DKR-3^T and its phylogenetically related members were below the species threshold values. Based on a polyphasic study, strain DKR-3^T represents a novel species belonging to the genus Cellulomonas, for which the name Cellulomonas fulva sp. nov. is proposed. The type strain is DKR-3^T (=KACC 22071^T=NBRC 114730^T).