Changes in autantio-obtusin and glucoaurantio-obtusin content in Cassiae Semen via treatment with a crude enzyme extract from Aspergillus usamii

Jong Moon Hur, Soon Ho Kwon, Jae Hyun So, Mira Jun, Young Hwa Kang, Yu Mi Lee, Kyung Bok Lee, In Koo Rhee, Moon Soon Lee, Kyung Sik Song

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Cassiae Semen (seeds of Cassia tora) showed a remarkably different HPLC chromatogram after being treated with a crude enzyme extract from Aspergillus usamii. Increased and decreased compounds were identified as aurantio-obtusin and glucoaurantio-obtusin, respectively. The aurantio-obtusin content reached its maximum level (133.58±0.39 μg/mg extract) after being incubated for 50 min at 37°C, whereas the inactivated crude enzyme-treated control remained unchanged (54.13±1.33 μg/mg). On the other hand, the glucoaurantio-obtusin content decreased by less than one-third (51.09±1.63 μg/mg) of the untreated control (143.19±2.12 μg/mg), suggesting that an increase in aurantio-obtusin content originated from the enzymatic cleavage of its glucoside glucoaurantio-obtusin.

Original languageEnglish
Pages (from-to)1894-1897
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume17
Issue number11
StatePublished - Nov 2007

Keywords

  • Aspergillus usamii
  • Aurantio-obtusin
  • Cassiae Semen
  • Enzyme treatment
  • Glucoaurantio-obtusin
  • Processes

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