Characteristics of ethylnitrosourea-induced cataracts

Min Jung Lee; Jae Young Kim; Yeun Jung Kim; Jae Woo Cho; Kyu Hyuk Cho; Chang Woo Song; Han Sung Jung

doi:10.1080/02713680902843047

Characteristics of ethylnitrosourea-induced cataracts

Min Jung Lee, Jae Young Kim, Yeun Jung Kim, Jae Woo Cho, Kyu Hyuk Cho, Chang Woo Song, Han Sung Jung

Department of Dentistry

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Abstract

Purpose: This study analyzed genes associated with the morphology and regulation of ethylnitrosourea (ENU)-induced cataract mouse. Materials and Methods: Immunohistochemistry analysis using anti-crystallins and PCNA antibody revealed that the localization pattern of these specific markers differed between the cataractous and wild-type lens epithelium. Two-dimensional electrophoresis and microarray techniques were used to identify the proteins and genes related to ENU-induced cataract. Results: A novel ENU-induced mutation in the mouse led to nuclear and cortical opacity of the eye lens at 5 weeks postnatal. This cataract phenotype was similar to that of the zonular-pulverulent type of human cataract. Crystallin proteins and gap-junction genes have relations to the formation of cataract. Conclusions: Together, the results suggest that various proteins affect the formation and specific phenotypes of ENU-induced cataract mouse.

Original language	English
Pages (from-to)	360-368
Number of pages	9
Journal	Current Eye Research
Volume	34
Issue number	5
DOIs	https://doi.org/10.1080/02713680902843047
State	Published - May 2009

Keywords

Cataract
Crystallins
ENU (ethylnitrosourea)
Gap junction
Zonular-pulverulent cataract type

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10.1080/02713680902843047

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title = "Characteristics of ethylnitrosourea-induced cataracts",

abstract = "Purpose: This study analyzed genes associated with the morphology and regulation of ethylnitrosourea (ENU)-induced cataract mouse. Materials and Methods: Immunohistochemistry analysis using anti-crystallins and PCNA antibody revealed that the localization pattern of these specific markers differed between the cataractous and wild-type lens epithelium. Two-dimensional electrophoresis and microarray techniques were used to identify the proteins and genes related to ENU-induced cataract. Results: A novel ENU-induced mutation in the mouse led to nuclear and cortical opacity of the eye lens at 5 weeks postnatal. This cataract phenotype was similar to that of the zonular-pulverulent type of human cataract. Crystallin proteins and gap-junction genes have relations to the formation of cataract. Conclusions: Together, the results suggest that various proteins affect the formation and specific phenotypes of ENU-induced cataract mouse.",

keywords = "Cataract, Crystallins, ENU (ethylnitrosourea), Gap junction, Zonular-pulverulent cataract type",

author = "Lee, {Min Jung} and Kim, {Jae Young} and Kim, {Yeun Jung} and Cho, {Jae Woo} and Cho, {Kyu Hyuk} and Song, {Chang Woo} and Jung, {Han Sung}",

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T1 - Characteristics of ethylnitrosourea-induced cataracts

AU - Lee, Min Jung

AU - Kim, Jae Young

AU - Kim, Yeun Jung

AU - Cho, Jae Woo

AU - Cho, Kyu Hyuk

AU - Song, Chang Woo

AU - Jung, Han Sung

PY - 2009/5

Y1 - 2009/5

N2 - Purpose: This study analyzed genes associated with the morphology and regulation of ethylnitrosourea (ENU)-induced cataract mouse. Materials and Methods: Immunohistochemistry analysis using anti-crystallins and PCNA antibody revealed that the localization pattern of these specific markers differed between the cataractous and wild-type lens epithelium. Two-dimensional electrophoresis and microarray techniques were used to identify the proteins and genes related to ENU-induced cataract. Results: A novel ENU-induced mutation in the mouse led to nuclear and cortical opacity of the eye lens at 5 weeks postnatal. This cataract phenotype was similar to that of the zonular-pulverulent type of human cataract. Crystallin proteins and gap-junction genes have relations to the formation of cataract. Conclusions: Together, the results suggest that various proteins affect the formation and specific phenotypes of ENU-induced cataract mouse.

AB - Purpose: This study analyzed genes associated with the morphology and regulation of ethylnitrosourea (ENU)-induced cataract mouse. Materials and Methods: Immunohistochemistry analysis using anti-crystallins and PCNA antibody revealed that the localization pattern of these specific markers differed between the cataractous and wild-type lens epithelium. Two-dimensional electrophoresis and microarray techniques were used to identify the proteins and genes related to ENU-induced cataract. Results: A novel ENU-induced mutation in the mouse led to nuclear and cortical opacity of the eye lens at 5 weeks postnatal. This cataract phenotype was similar to that of the zonular-pulverulent type of human cataract. Crystallin proteins and gap-junction genes have relations to the formation of cataract. Conclusions: Together, the results suggest that various proteins affect the formation and specific phenotypes of ENU-induced cataract mouse.

KW - Cataract

KW - Crystallins

KW - ENU (ethylnitrosourea)

KW - Gap junction

KW - Zonular-pulverulent cataract type

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Characteristics of ethylnitrosourea-induced cataracts

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Keywords

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