Characteristics of mushroom phellinus baumii extracts with enzyme pretreatment

This study was conducted to establish optimized β-glucan extraction method through enzymatic hydrolysis from Phellinus baumii and investigate β-glucan contents and physicochemical properties. The optimal condition was obtained with the enzyme concentration of 0.66% (v/v), reaction time of 6.08 h (R²=0.9245) and the β-glucan contents from the Phellinus baumii extracts under the optimized condition was 1.9594 g/100 g. β-Glucan yield (0.76-16.40%) of enzyme beta-glucan extract (EBE) was three fold higher than that of non-enzyme beta-glucan extract (NEBE). β-Glucan purity (11.15-59.05%) of non-enzyme beta-glucan (NEB) and that of enzyme beta-glucan (EB) were higher than that of NEBE and that of EBE. β-Glucan purity of EB (59.05%) and β-glucan contents of EB (3.38 g/100 g) showed higher than those of others. Total sugar contents (0.61-1.17 mg/ mL) showed that NEB and EB were higher than that of NEBE and EBE, EB had the highest total sugar content as 1.17 mg/mL, respectively. Protein contents (0.44-11.73 mg/mL) of NEBE and that of EBE were higher than that of NEB, that of EB. In FT-IR spectrum, the band at 890 cm⁻¹ of microcapsule was attributed to a β-1,3-glucan. The toxicities of β-glucan from Phellinus baumii in both melanoma cell lines was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoli um bromide assay and β-glucan from Phellinus baumii has no toxicity until 30 μg/mL. The effects of β-glucan from Phellinus baumii on inhibition of cancer cell proliferation were detected by using a wound healing assay. The effect of NEB and EB were higher than NEBE and EBE, especially 30 μg/mL of EB had the highest in both melanoma cell lines.