Characterization of β-glucan extracts and purified extracts from medicinal mushrooms and their biological activities

Phellinus baumii and Ganoderma lucidum are the representative medicinal mushrooms in Korea. The β-glucan extract was prepared from these mushrooms, and their physicochemical characteristics, antioxidant contents, antioxidant activities, cell viability, and inhibition activities of cancer cell migration were investigated. The optimum β-glucan extraction condition was 6% (v/w) concentration of enzyme (Viscozyme L), 50:50 (w/w) mushroom ratio, and 8 hours of reaction time, respectively. The β-glucan purity of PGBEP (Phellinus baumii and Ganoderma lucidum β-glucan extract with purification) was higher than of PGBE (Phellinus baumii and Ganoderma lucidum β-glucan extract). In contrast, the extraction yield and β-glucan contents of PGBEP were lower than of PGBE (p<0.05). The FT-IR spectrum of PGBE and PGBEP showed that α-glycosidic and β-glycosidic linkage formation was determined by the appearance of the peaks at 890-822 cm^-1 range. The antioxidant contents and antioxidant activities of PGBE were higher than of PGBEP. The cell viability by MTT assay of PGBE and PGBEP was higher than 80%. The inhibition activity of cancer cell migration by wound healing assay of PGBEP was significantly higher than of PGBE and positive control.