Characterization of a brain tumor cell line established from transgenic mice expressing the vasopressin SV-40 T antigen

Hyun Kim Sung, Ok Kim Myoung, Ryeul Lee Sang, Soo Kim Kil, Tae Hoon Lee, Taek Lee Hoon, Hong Ha Ji, Yoon Kim Tae, Young Ryoo Zae

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We previously reported that transgenic mice produced with a transgene consisting of the SV40 T antigen and vasopressin without the 3′-flanking region exhibit brain tumors and lymphoma. In this study, transgenic mice were produced with the fusion gene containing the SV40 T antigen and the whole vasopressin gene with the 3′-flanking region. Six transgenic mice were generated, five which died after 2-6 weeks. The remaining founder mouse was investigated for fusion gene expression and tumor progression at the age of 6 weeks. Brain tumor cells were characterized for phenotypes and transgene expression. During in vitro cell cultures, the phenotypic appearances at 10, 20, and 30 passages were as a uniform monolayer with similar growth rates. The site of SV40 T antigen integration was in the A2 region of chromosome 11, and SV40 T antigen was expressed at the same level in cells of both earlier and later passages. Thirty passages were probably insufficient to reach crisis and immortalization. These cells enriched brain tumor cell compositions with astrocytes and neuronal cells.

Original languageEnglish
Pages (from-to)196-202
Number of pages7
JournalExperimental and Molecular Medicine
Volume38
Issue number3
DOIs
StatePublished - 30 Jun 2006

Keywords

  • Astrocyte cell
  • Brain tumor cell
  • Neuronal cell
  • Transgenic mice
  • Vasopressin

Fingerprint

Dive into the research topics of 'Characterization of a brain tumor cell line established from transgenic mice expressing the vasopressin SV-40 T antigen'. Together they form a unique fingerprint.

Cite this