Characterization of dwarf and narrow leaf (dnl-4) mutant in rice

Ki Deuk Bae, Tae Young Um, Won Tae Yang, Tae Hyeon Park, So Yeon Hong, Kyung Min Kim, Young Soo Chung, Dae Jin Yun, Doh Hoon Kim

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Height and leaf morphology are important agronomic traits of the major crop plant rice (Oryza sativa). In previous studies, the dwarf and narrow leaf genes (dnl1, dnl2 and dnl3) have identified in rice. Using the Ac/Ds knockout system, we found a new dwarf and narrow leaf (dnl) mutant and identified mutated gene. The dnl-4 mutant showed reduced plant height and leaf blade width compared to the wild type, and increased leaf inclination. The morphological defects of the mutant were caused by the suppressed expression of the DNL-4 gene, which encodes a pfkB carbohydrate kinase protein. These results suggest that DNL-4 expression is involved in modulating plant height and leaf growth. Furthermore, DNL-4 expression also affects productivity in rice: the dnl-4 mutant exhibited reduced panicle length and grain width compared with the wild type. To understand DNL-4 function in rice, we analyzed the expression levels of leaf growth-related genes, such as NAL1, NAL7, and CSLD4, in the dnl-4 mutant. Expression of NAL1 and NAL7 was downregulated in the dnl-4 mutant compared to the wild type. The observation that DNL-4 expression corresponded with that of NAL1 and NAL7 is consistent with the narrow leaf phenotype of the dnl-4 mutant. These results suggest that DNL-4 regulates plant height and leaf structure in rice.

Original languageEnglish
Article number1849490
JournalPlant Signaling and Behavior
Volume16
Issue number2
DOIs
StatePublished - 2021

Keywords

  • Dwarf and narrow leaf mutant
  • Oryza sativa
  • pfkB carbohydrate kinase protein

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