Characterization of fatty acid components from Tetradesmus obliquus KNUA019 (Chlorophyta, Scenedesmaceae) for a resource of biofuel production

Green algae produce fatty acid components that can be used in biofuel production without the need for additional nutrients. We aimed to elucidate the contribution of the T. obliquus KNUA019 through the control of the optimal culture (solvent, culture time, nitrogen, and phosphorus) conditions and thermal analysis (DTA and TGA curves) that affects fatty acid productivity maximum lipid yields from the four culture methods. Phylogenetic analysis of Tetradesmus obliquus (Turpin), Scenedesmus obliquus (Turpin), Acutodesmus obliquus (Turpin), and Chlorella sorokiniana (Shihira & R.W.Krauss) strains was attempted using the internal transcribed spacer. T. obliquus KNUA019 can produce significant amounts of carbon-containing components, which are valuable for use as energy sources. As a result of GC analysis, T. obliquus KNUA019 generates fatty acid components that are directly useful as biofuels, such as tetradecanoic acid (C₁₄H₂₈O₂), methyl Z-11-tetradecenoate (C₁₅H₂₈O₂), tetradecanoic acid (C₁₅H₃₀O₂), 9-hexadecenoic acid (C₁₅H₃₀O₂), pentadecane (C₁₅H₃₂), 8-heptadecene (C₁₇H₃₄), hexadecanoic acid (C₁₇H₃₄O₂), heptadecane (C₁₇H₃₆), 9-octadecenoic acid (C₁₉H₃₆O₂), octadecanoic acid (C₁₉H₃₈O₂), and 3,7,11,15-tetramethyl-2-hexadecen-1-ol (C₂₀H₄₀O). These fatty acids can be used directly as biofuel precursors without transesterification. We indicate that commercial biofuel production is possible using mass culture of T. obliquus KNUA019, reducing production costs. This process was indicated as an optimum method for simplifying the process of fatty acid components under optimal culture conditions for a source of biofuels.