TY - JOUR
T1 - Characterization of sodium laureth sulfate by reversed-phase liquid chromatography with evaporative light scattering detection and 1H nuclear magnetic resonance spectroscopy
AU - Im, Sung Hyun
AU - Ryoo, Jae Jeong
PY - 2009/3/20
Y1 - 2009/3/20
N2 - A direct and effective method utilizing reversed-phase liquid chromatography combined with evaporative light scattering detection was developed to determine the relative ratio of different alkyl chain lengths, to quantify the average ethylene oxide (EO) level and to identify EO distribution in the presence of sodium laureth sulfates. A C8 bonded silica gel column and an acetonitrile-water gradient mobile phase containing ammonium acetate were used as the best stationary and mobile phase, respectively. The results were confirmed by nuclear magnetic resonance spectroscopy. The detection limit was 80 μg/mL and the calibration curve, i.e., the log-log plots (peak area vs. concentration), was linear in the working range of 80-4200 μg/mL with R2 values of above 0.999 (in the case of 3 mol sodium laureth sulfates). Furthermore, the application of the chromatographic method to a commercial product without pretreatment was presented. The raw material was identified by fast atom bombardment mass spectrometry.
AB - A direct and effective method utilizing reversed-phase liquid chromatography combined with evaporative light scattering detection was developed to determine the relative ratio of different alkyl chain lengths, to quantify the average ethylene oxide (EO) level and to identify EO distribution in the presence of sodium laureth sulfates. A C8 bonded silica gel column and an acetonitrile-water gradient mobile phase containing ammonium acetate were used as the best stationary and mobile phase, respectively. The results were confirmed by nuclear magnetic resonance spectroscopy. The detection limit was 80 μg/mL and the calibration curve, i.e., the log-log plots (peak area vs. concentration), was linear in the working range of 80-4200 μg/mL with R2 values of above 0.999 (in the case of 3 mol sodium laureth sulfates). Furthermore, the application of the chromatographic method to a commercial product without pretreatment was presented. The raw material was identified by fast atom bombardment mass spectrometry.
KW - Evaporative light scattering detection
KW - Nuclear magnetic resonance spectroscopy
KW - Reversed-phase liquid chromatography
KW - Sodium laureth sulfates
UR - http://www.scopus.com/inward/record.url?scp=60649096249&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2009.01.005
DO - 10.1016/j.chroma.2009.01.005
M3 - Article
C2 - 19185870
AN - SCOPUS:60649096249
SN - 0021-9673
VL - 1216
SP - 2339
EP - 2344
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 12
ER -