TY - JOUR
T1 - Characterization of the denitrification-associated phosphorus uptake properties of "Candidatusaccumulibacter phosphatis" clades in sludge subjected to enhanced biological phosphorus removal
AU - Kim, Jeong Myeong
AU - Lee, Hyo Jung
AU - Lee, Dae Sung
AU - Jeon, Che Ok
PY - 2013/3
Y1 - 2013/3
N2 - To characterize the denitrifying phosphorus (P) uptake properties of ""CandidatusAccumulibacter phosphatis," a sequencing batch reactor (SBR) was operated with acetate. The SBR operation was gradually acclimated from anaerobic-oxic (AO) to anaer-obic-anoxic-oxic (A2O) conditions by stepwise increases of nitrate concentration and the anoxic time. The communities of "Ca. Accumulibacter" and associated bacteria at the initial (AO) and final (A2O) stages were compared using 16S rRNA and polyphosphate kinase genes and using fluorescencein situhybridization (FISH). The acclimation process led to a clear shift in the relative abundances of recognized "Ca.Accumulibacter" subpopulations from clades IIA>IA>IIF to clades IIC>IA> IIF, as well as to increases in the abundance of other associated bacteria (Dechloromonas[from 1.2% to 19.2%] and "Candidatus Competibacter phosphatis" [from 16.4% to 20.0%]), while the overall "Ca.Accumulibacter" abundance decreased (from 55.1% to 29.2%). A series of batch experiments combined with FISH/microautoradiography (MAR) analyses was performed to charac-terize the denitrifying P uptake properties of the "Ca.Accumulibacter" clades. In FISH/MAR experiments using slightly diluted sludge (-0.5 g/liter), all "Ca.Accumulibacter" clades successfully took up phosphorus in the presence of nitrate. However, the "Ca.Accumulibacter" clades showed no P uptake in the presence of nitrate when the sludge was highly diluted (0.005 g/liter); under these conditions, reduction of nitrate to nitrite did not occur, whereas P uptake by "Ca.Accumulibacter" clades occurred when nitrite was added. These results suggest that the "Ca.Accumulibacter" cells lack nitrate reduction capabilities and that P uptake by "Ca.Accumulibacter" is dependent upon nitrite generated by associated nitrate-reducing bacteria such asDechlo-romonasand "Ca.Competibacter."
AB - To characterize the denitrifying phosphorus (P) uptake properties of ""CandidatusAccumulibacter phosphatis," a sequencing batch reactor (SBR) was operated with acetate. The SBR operation was gradually acclimated from anaerobic-oxic (AO) to anaer-obic-anoxic-oxic (A2O) conditions by stepwise increases of nitrate concentration and the anoxic time. The communities of "Ca. Accumulibacter" and associated bacteria at the initial (AO) and final (A2O) stages were compared using 16S rRNA and polyphosphate kinase genes and using fluorescencein situhybridization (FISH). The acclimation process led to a clear shift in the relative abundances of recognized "Ca.Accumulibacter" subpopulations from clades IIA>IA>IIF to clades IIC>IA> IIF, as well as to increases in the abundance of other associated bacteria (Dechloromonas[from 1.2% to 19.2%] and "Candidatus Competibacter phosphatis" [from 16.4% to 20.0%]), while the overall "Ca.Accumulibacter" abundance decreased (from 55.1% to 29.2%). A series of batch experiments combined with FISH/microautoradiography (MAR) analyses was performed to charac-terize the denitrifying P uptake properties of the "Ca.Accumulibacter" clades. In FISH/MAR experiments using slightly diluted sludge (-0.5 g/liter), all "Ca.Accumulibacter" clades successfully took up phosphorus in the presence of nitrate. However, the "Ca.Accumulibacter" clades showed no P uptake in the presence of nitrate when the sludge was highly diluted (0.005 g/liter); under these conditions, reduction of nitrate to nitrite did not occur, whereas P uptake by "Ca.Accumulibacter" clades occurred when nitrite was added. These results suggest that the "Ca.Accumulibacter" cells lack nitrate reduction capabilities and that P uptake by "Ca.Accumulibacter" is dependent upon nitrite generated by associated nitrate-reducing bacteria such asDechlo-romonasand "Ca.Competibacter."
UR - http://www.scopus.com/inward/record.url?scp=84874816824&partnerID=8YFLogxK
U2 - 10.1128/AEM.03464-12
DO - 10.1128/AEM.03464-12
M3 - Article
C2 - 23335771
AN - SCOPUS:84874816824
SN - 0099-2240
VL - 79
SP - 1969
EP - 1979
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 6
ER -