Characterization of transgenic rice plants expressing an Arabidopsis FAD7

J. Song, D. E. Lee, S. Jung, H. Kim, O. Han, B. H. Cho, I. J. Lee, K. Back

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Fatty acid ω-3 desaturase (FAD) is the key enzyme catalyzing the formation of trienoic fatty acids. We utilized an Arabidopsis FAD7 gene and the seven independent transgenic rice plants harbouring 1 to 3 copies of this gene were generated. The expression of FAD7 mRNA was different among independent transgenic lines regardless of the copy number. The total linolenic acid (18:3) contents reduced by about 7 - 32 % in transgenic rice plants but the linoleic acid (18:2) content increased accordingly. With or without wounding treatments, the jasmonate content was higher in transgenic lines than in wild-type rice plant. The transgenic lines overproducing jasmonate also showed increased expression of PR1b mRNA and allene oxide synthase inresponse to wounding.

Original languageEnglish
Pages (from-to)361-366
Number of pages6
JournalBiologia Plantarum
Volume48
Issue number3
DOIs
StatePublished - 2004

Keywords

  • fatty acid desaturase
  • jasmonic acid
  • linoleic acid
  • linolenic acid
  • malondialdehyde

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