Abstract
Fatty acid ω-3 desaturase (FAD) is the key enzyme catalyzing the formation of trienoic fatty acids. We utilized an Arabidopsis FAD7 gene and the seven independent transgenic rice plants harbouring 1 to 3 copies of this gene were generated. The expression of FAD7 mRNA was different among independent transgenic lines regardless of the copy number. The total linolenic acid (18:3) contents reduced by about 7 - 32 % in transgenic rice plants but the linoleic acid (18:2) content increased accordingly. With or without wounding treatments, the jasmonate content was higher in transgenic lines than in wild-type rice plant. The transgenic lines overproducing jasmonate also showed increased expression of PR1b mRNA and allene oxide synthase inresponse to wounding.
Original language | English |
---|---|
Pages (from-to) | 361-366 |
Number of pages | 6 |
Journal | Biologia Plantarum |
Volume | 48 |
Issue number | 3 |
DOIs | |
State | Published - 2004 |
Keywords
- fatty acid desaturase
- jasmonic acid
- linoleic acid
- linolenic acid
- malondialdehyde