Chemical constituents from the fruits of Ligustrum japonicum and their inhibitory effects on T cell activation

Quynh Mai Thi Ngo; Hyun Su Lee; Van Thu Nguyen; Jeong Ah Kim; Mi Hee Woo; Byung Sun Min

doi:10.1016/j.phytochem.2017.06.001

Chemical constituents from the fruits of Ligustrum japonicum and their inhibitory effects on T cell activation

Quynh Mai Thi Ngo, Hyun Su Lee, Van Thu Nguyen, Jeong Ah Kim, Mi Hee Woo, Byung Sun Min

Catholic University of Daegu

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

A previously undescribed nor-dammarane, 3β,20,23-trihydroxy-24,25,26,27-tetranordammarane; three previously undescribed secoiridoid glycosides, ligujaponosides A–B, and iso-oleonuzhenide; and twenty three known compounds, were isolated from the fruits of Ligustrum japonicum Thunb. Their chemical structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, and HRMS. The isolated compounds were screened for immunosuppressive effects on T activated cells by evaluating interleukin-2 (IL-2) production. Among them, sesamin inhibited IL-2 production in Jurkat T cells with an IC₅₀ value of 38 ± 2 μM. In addition, sesamin inhibited the phosphorylation of extracellular signal-regulated protein kinase (ERK), a member of the mitogen-activated protein kinase (MAPK) family, in phorbol 12-myristate 13-acetate (PMA)/A23187-stimulated T cells. Therefore, sesamin was demonstrated to inhibit T cell activation via regulation of MAPK phosphorylation pathway.

Original language	English
Pages (from-to)	147-155
Number of pages	9
Journal	Phytochemistry
Volume	141
DOIs	https://doi.org/10.1016/j.phytochem.2017.06.001
State	Published - Sep 2017

Keywords

Ligustrum japonicum
Oleaceae
Secoiridoid
Sesamin
T cell activation
Triterpenoid

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10.1016/j.phytochem.2017.06.001

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@article{358603f803794dd6a6358c02a003ecdb,

title = "Chemical constituents from the fruits of Ligustrum japonicum and their inhibitory effects on T cell activation",

abstract = "A previously undescribed nor-dammarane, 3β,20,23-trihydroxy-24,25,26,27-tetranordammarane; three previously undescribed secoiridoid glycosides, ligujaponosides A–B, and iso-oleonuzhenide; and twenty three known compounds, were isolated from the fruits of Ligustrum japonicum Thunb. Their chemical structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, and HRMS. The isolated compounds were screened for immunosuppressive effects on T activated cells by evaluating interleukin-2 (IL-2) production. Among them, sesamin inhibited IL-2 production in Jurkat T cells with an IC50 value of 38 ± 2 μM. In addition, sesamin inhibited the phosphorylation of extracellular signal-regulated protein kinase (ERK), a member of the mitogen-activated protein kinase (MAPK) family, in phorbol 12-myristate 13-acetate (PMA)/A23187-stimulated T cells. Therefore, sesamin was demonstrated to inhibit T cell activation via regulation of MAPK phosphorylation pathway.",

keywords = "Ligustrum japonicum, Oleaceae, Secoiridoid, Sesamin, T cell activation, Triterpenoid",

author = "Ngo, {Quynh Mai Thi} and Lee, {Hyun Su} and Nguyen, {Van Thu} and Kim, {Jeong Ah} and Woo, {Mi Hee} and Min, {Byung Sun}",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2017",

month = sep,

doi = "10.1016/j.phytochem.2017.06.001",

language = "English",

volume = "141",

pages = "147--155",

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T1 - Chemical constituents from the fruits of Ligustrum japonicum and their inhibitory effects on T cell activation

AU - Ngo, Quynh Mai Thi

AU - Lee, Hyun Su

AU - Nguyen, Van Thu

AU - Kim, Jeong Ah

AU - Woo, Mi Hee

AU - Min, Byung Sun

PY - 2017/9

Y1 - 2017/9

N2 - A previously undescribed nor-dammarane, 3β,20,23-trihydroxy-24,25,26,27-tetranordammarane; three previously undescribed secoiridoid glycosides, ligujaponosides A–B, and iso-oleonuzhenide; and twenty three known compounds, were isolated from the fruits of Ligustrum japonicum Thunb. Their chemical structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, and HRMS. The isolated compounds were screened for immunosuppressive effects on T activated cells by evaluating interleukin-2 (IL-2) production. Among them, sesamin inhibited IL-2 production in Jurkat T cells with an IC50 value of 38 ± 2 μM. In addition, sesamin inhibited the phosphorylation of extracellular signal-regulated protein kinase (ERK), a member of the mitogen-activated protein kinase (MAPK) family, in phorbol 12-myristate 13-acetate (PMA)/A23187-stimulated T cells. Therefore, sesamin was demonstrated to inhibit T cell activation via regulation of MAPK phosphorylation pathway.

AB - A previously undescribed nor-dammarane, 3β,20,23-trihydroxy-24,25,26,27-tetranordammarane; three previously undescribed secoiridoid glycosides, ligujaponosides A–B, and iso-oleonuzhenide; and twenty three known compounds, were isolated from the fruits of Ligustrum japonicum Thunb. Their chemical structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, and HRMS. The isolated compounds were screened for immunosuppressive effects on T activated cells by evaluating interleukin-2 (IL-2) production. Among them, sesamin inhibited IL-2 production in Jurkat T cells with an IC50 value of 38 ± 2 μM. In addition, sesamin inhibited the phosphorylation of extracellular signal-regulated protein kinase (ERK), a member of the mitogen-activated protein kinase (MAPK) family, in phorbol 12-myristate 13-acetate (PMA)/A23187-stimulated T cells. Therefore, sesamin was demonstrated to inhibit T cell activation via regulation of MAPK phosphorylation pathway.

KW - Ligustrum japonicum

KW - Oleaceae

KW - Secoiridoid

KW - Sesamin

KW - T cell activation

KW - Triterpenoid

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U2 - 10.1016/j.phytochem.2017.06.001

DO - 10.1016/j.phytochem.2017.06.001

M3 - Article

C2 - 28618355

AN - SCOPUS:85020432754

SN - 0031-9422

VL - 141

SP - 147

EP - 155

JO - Phytochemistry

JF - Phytochemistry

ER -

Chemical constituents from the fruits of Ligustrum japonicum and their inhibitory effects on T cell activation

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