Chitosan-functionalized supermagnetic halloysite nanotubes for covalent laccase immobilization

Avinash A. Kadam, Jiseon Jang, Seung Cheol Jee, Jung Suk Sung, Dae Sung Lee

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Halloysite nanotubes (HNTs) were modified with supermagnetic Fe3O4 (M-HNTs) and functionalized with chitosan (CTA) (termed as M-HNTs–CTA). Furthermore, M-HNTs–CTA were cross-linked using glutaraldehyde and applied for covalent laccase immobilization (M-HNTs–CTA–Lac). Facile-synthesized modified HNTs were structurally characterized by scanning electron microscopy, high resolution transmission electron microscopy, X-ray photoelectron spectroscopy, and thermogravimetric analyses. M-HNTs–CTA–Lac exhibited 92.74 mg/g of laccase immobilization capacity and 92% of activity recovery. Biochemical properties of M-HNTs–CTA–Lac exhibited higher pH and temperature stabilities, with exceptional reusability capabilities until the 11th cycle. Moreover, M-HNTs–CTA–Lac exhibited 87% of 2,2′-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)-mediated Direct Red 80 (DR80) decolorization. By the 11th cycle, M-HNTs–CTA–Lac exhibited 33% DR80 decolorization. Therefore, M-HNTs–CTA can function as CTA-modified supermagnetic nonreactors for immobilization of biomacromolecules. The investigated M-HNTs–CTA–Lac are thus biocompatible and environment-friendly biocatalysts for degradation of textile waste, such as DR80, and can be rapidly retrieved from aqueous solution by a magnet after decontamination of environmental pollutants.

Original languageEnglish
Pages (from-to)208-216
Number of pages9
JournalCarbohydrate Polymers
Volume194
DOIs
StatePublished - 15 Aug 2018

Keywords

  • Chitosan
  • Halloysite nanotubes
  • Laccase
  • Laccase-loaded nanotubular reactors
  • Nanobiocatalysis
  • Sustainable environmental cleanup

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