Chlorpyrifos degradation efficiency of Bacillus sp. laccase immobilized on iron magnetic nanoparticles

P. Srinivasan; T. Selvankumar; Bilal Ahamad Paray; Muneeb U. Rehman; S. Kamala-Kannan; M. Govarthanan; Woong Kim; K. Selvam

doi:10.1007/s13205-020-02363-6

Chlorpyrifos degradation efficiency of Bacillus sp. laccase immobilized on iron magnetic nanoparticles

P. Srinivasan, T. Selvankumar, Bilal Ahamad Paray, Muneeb U. Rehman, S. Kamala-Kannan, M. Govarthanan, Woong Kim, K. Selvam

Department of Environmental Engineering

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The present study explored the immobilization of laccase onto iron magnetic nanoparticles (MNPs) to enhance its enzymatic properties and applications. The immobilization process was optimized using Box–Behnken design (BBD). BBD showed significance towards the quadratic model with experimental data. Maximum laccase activity recovery (99%) of the predicted model was observed at 0.75 mg/mL of laccase concentration, 200 mg/mL of MNPs, 0.3% cross linking with carbodiimide, and 3 h of cross-linking time. The magnetization activity of MNPs (8 emu/g) and the immobilized laccase with MNPs (4 emu/g) was analyzed using vibrating sample magnetometer (VSM). Maximum activity of immobilized laccase was observed at pH 7.0 and 55 °C. The immobilized laccase has greater stability (100 h) and significant chlorpyrifos (pesticide) degradation activity. High-performance liquid chromatography (HPLC) results confirmed the degraded metabolic products of chlorpyrifos. In all, the immobilized laccase was superior to free laccase, showing promising structural and application characteristics.

Original language	English
Article number	366
Journal	3 Biotech
Volume	10
Issue number	8
DOIs	https://doi.org/10.1007/s13205-020-02363-6
State	Published - 1 Aug 2020

Keywords

Immobilization
Laccase
Magnetic nanoparticles
Optimization
Pesticides

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title = "Chlorpyrifos degradation efficiency of Bacillus sp. laccase immobilized on iron magnetic nanoparticles",

abstract = "The present study explored the immobilization of laccase onto iron magnetic nanoparticles (MNPs) to enhance its enzymatic properties and applications. The immobilization process was optimized using Box–Behnken design (BBD). BBD showed significance towards the quadratic model with experimental data. Maximum laccase activity recovery (99%) of the predicted model was observed at 0.75 mg/mL of laccase concentration, 200 mg/mL of MNPs, 0.3% cross linking with carbodiimide, and 3 h of cross-linking time. The magnetization activity of MNPs (8 emu/g) and the immobilized laccase with MNPs (4 emu/g) was analyzed using vibrating sample magnetometer (VSM). Maximum activity of immobilized laccase was observed at pH 7.0 and 55 °C. The immobilized laccase has greater stability (100 h) and significant chlorpyrifos (pesticide) degradation activity. High-performance liquid chromatography (HPLC) results confirmed the degraded metabolic products of chlorpyrifos. In all, the immobilized laccase was superior to free laccase, showing promising structural and application characteristics.",

keywords = "Immobilization, Laccase, Magnetic nanoparticles, Optimization, Pesticides",

author = "P. Srinivasan and T. Selvankumar and Paray, {Bilal Ahamad} and Rehman, {Muneeb U.} and S. Kamala-Kannan and M. Govarthanan and Woong Kim and K. Selvam",

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AU - Srinivasan, P.

AU - Selvankumar, T.

AU - Paray, Bilal Ahamad

AU - Rehman, Muneeb U.

AU - Kamala-Kannan, S.

AU - Govarthanan, M.

AU - Kim, Woong

AU - Selvam, K.

PY - 2020/8/1

Y1 - 2020/8/1

N2 - The present study explored the immobilization of laccase onto iron magnetic nanoparticles (MNPs) to enhance its enzymatic properties and applications. The immobilization process was optimized using Box–Behnken design (BBD). BBD showed significance towards the quadratic model with experimental data. Maximum laccase activity recovery (99%) of the predicted model was observed at 0.75 mg/mL of laccase concentration, 200 mg/mL of MNPs, 0.3% cross linking with carbodiimide, and 3 h of cross-linking time. The magnetization activity of MNPs (8 emu/g) and the immobilized laccase with MNPs (4 emu/g) was analyzed using vibrating sample magnetometer (VSM). Maximum activity of immobilized laccase was observed at pH 7.0 and 55 °C. The immobilized laccase has greater stability (100 h) and significant chlorpyrifos (pesticide) degradation activity. High-performance liquid chromatography (HPLC) results confirmed the degraded metabolic products of chlorpyrifos. In all, the immobilized laccase was superior to free laccase, showing promising structural and application characteristics.

AB - The present study explored the immobilization of laccase onto iron magnetic nanoparticles (MNPs) to enhance its enzymatic properties and applications. The immobilization process was optimized using Box–Behnken design (BBD). BBD showed significance towards the quadratic model with experimental data. Maximum laccase activity recovery (99%) of the predicted model was observed at 0.75 mg/mL of laccase concentration, 200 mg/mL of MNPs, 0.3% cross linking with carbodiimide, and 3 h of cross-linking time. The magnetization activity of MNPs (8 emu/g) and the immobilized laccase with MNPs (4 emu/g) was analyzed using vibrating sample magnetometer (VSM). Maximum activity of immobilized laccase was observed at pH 7.0 and 55 °C. The immobilized laccase has greater stability (100 h) and significant chlorpyrifos (pesticide) degradation activity. High-performance liquid chromatography (HPLC) results confirmed the degraded metabolic products of chlorpyrifos. In all, the immobilized laccase was superior to free laccase, showing promising structural and application characteristics.

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KW - Laccase

KW - Magnetic nanoparticles

KW - Optimization

KW - Pesticides

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Chlorpyrifos degradation efficiency of Bacillus sp. laccase immobilized on iron magnetic nanoparticles

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