Cholesterol-linked pyrene excimer molecular beacon with enhanced cell permeability

Hyun S.eok Jeong; Young J.un Seo; Eun Kyung Bang; Gil T.ae Hwang; Jong H.a. Jung; Sung K.ey Jang; Byeang H.yean Kim

doi:10.1093/nass/nrn177

Cholesterol-linked pyrene excimer molecular beacon with enhanced cell permeability

Hyun S.eok Jeong, Young J.un Seo, Eun Kyung Bang, Gil T.ae Hwang, Jong H.a. Jung, Sung K.ey Jang, Byeang H.yean Kim

Pohang University of Science and Technology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Covelently labeled pyrene excimer molecular beacon (MB) with cholesterol moiety has been developed for enhanced the cellular delivery of MB.(1) Pyrene units were covalently attached into adenosine and incorporated to oligonucleotides at the complementary locations in opposite strands in the middle positions of hairpin stems. The system behaves as an effective MB that changes color from green to blue upon duplex formation. A cholesterol unit was also attached into a free terminus of one of these hairpins. The cholesterol-linked MBs enhanced the cellular delivery of the MBs and showed similar cell permeability to conventional transfection methods. These structurally simple cholesterol-based MB systems, which can be synthesized very efficiently, have good potential for opening up new and exciting opportunities in the field of in vivo biosensors.

Original language	English
Pages (from-to)	351-352
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	52
DOIs	https://doi.org/10.1093/nass/nrn177
State	Published - 2008

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title = "Cholesterol-linked pyrene excimer molecular beacon with enhanced cell permeability",

abstract = "Covelently labeled pyrene excimer molecular beacon (MB) with cholesterol moiety has been developed for enhanced the cellular delivery of MB.(1) Pyrene units were covalently attached into adenosine and incorporated to oligonucleotides at the complementary locations in opposite strands in the middle positions of hairpin stems. The system behaves as an effective MB that changes color from green to blue upon duplex formation. A cholesterol unit was also attached into a free terminus of one of these hairpins. The cholesterol-linked MBs enhanced the cellular delivery of the MBs and showed similar cell permeability to conventional transfection methods. These structurally simple cholesterol-based MB systems, which can be synthesized very efficiently, have good potential for opening up new and exciting opportunities in the field of in vivo biosensors.",

author = "Jeong, {Hyun S.eok} and Seo, {Young J.un} and Bang, {Eun Kyung} and Hwang, {Gil T.ae} and Jung, {Jong H.a.} and Jang, {Sung K.ey} and Kim, {Byeang H.yean}",

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T1 - Cholesterol-linked pyrene excimer molecular beacon with enhanced cell permeability

AU - Jeong, Hyun S.eok

AU - Seo, Young J.un

AU - Bang, Eun Kyung

AU - Hwang, Gil T.ae

AU - Jung, Jong H.a.

AU - Jang, Sung K.ey

AU - Kim, Byeang H.yean

PY - 2008

Y1 - 2008

N2 - Covelently labeled pyrene excimer molecular beacon (MB) with cholesterol moiety has been developed for enhanced the cellular delivery of MB.(1) Pyrene units were covalently attached into adenosine and incorporated to oligonucleotides at the complementary locations in opposite strands in the middle positions of hairpin stems. The system behaves as an effective MB that changes color from green to blue upon duplex formation. A cholesterol unit was also attached into a free terminus of one of these hairpins. The cholesterol-linked MBs enhanced the cellular delivery of the MBs and showed similar cell permeability to conventional transfection methods. These structurally simple cholesterol-based MB systems, which can be synthesized very efficiently, have good potential for opening up new and exciting opportunities in the field of in vivo biosensors.

AB - Covelently labeled pyrene excimer molecular beacon (MB) with cholesterol moiety has been developed for enhanced the cellular delivery of MB.(1) Pyrene units were covalently attached into adenosine and incorporated to oligonucleotides at the complementary locations in opposite strands in the middle positions of hairpin stems. The system behaves as an effective MB that changes color from green to blue upon duplex formation. A cholesterol unit was also attached into a free terminus of one of these hairpins. The cholesterol-linked MBs enhanced the cellular delivery of the MBs and showed similar cell permeability to conventional transfection methods. These structurally simple cholesterol-based MB systems, which can be synthesized very efficiently, have good potential for opening up new and exciting opportunities in the field of in vivo biosensors.

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DO - 10.1093/nass/nrn177

M3 - Article

C2 - 18776398

AN - SCOPUS:78649416308

SN - 1746-8272

SP - 351

EP - 352

JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

IS - 52

ER -

Cholesterol-linked pyrene excimer molecular beacon with enhanced cell permeability

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