Cloning and characterization of a bovine alpha interferon receptor

Jin Kyu Lim; Jerome A. Langer

doi:10.1016/0167-4781(93)90129-2

Cloning and characterization of a bovine alpha interferon receptor

Jin Kyu Lim, Jerome A. Langer

School of Food Science & Biotechnology

Rutgers - The State University of New Jersey, New Brunswick

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A bovine interferon α receptor (BoIFN-αR1) cDNA, homologous to the human cDNA [1], was isolated. Transfection of the BoIFN-αR1 cDNA into monkey COS cells results in a large increase in high-affinity binding sites for human IFN-αA and IFN-αB. Covalent crosslinking of radiolabeled HuIFN-αA and -αB demonstrates that the complex of [³²P]HuIFN with the BoIFN-αR1 protein (predicted mass, 61375) expressed in COS cells migrates as a 140-150 kDa band.

Original language	English
Pages (from-to)	314-319
Number of pages	6
Journal	BBA - Gene Structure and Expression
Volume	1173
Issue number	3
DOIs	https://doi.org/10.1016/0167-4781(93)90129-2
State	Published - 25 Jun 1993

Keywords

cDNA sequence
Interferon receptor
Protein crosslinking
α-Interferon

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10.1016/0167-4781(93)90129-2

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title = "Cloning and characterization of a bovine alpha interferon receptor",

abstract = "A bovine interferon α receptor (BoIFN-αR1) cDNA, homologous to the human cDNA [1], was isolated. Transfection of the BoIFN-αR1 cDNA into monkey COS cells results in a large increase in high-affinity binding sites for human IFN-αA and IFN-αB. Covalent crosslinking of radiolabeled HuIFN-αA and -αB demonstrates that the complex of [32P]HuIFN with the BoIFN-αR1 protein (predicted mass, 61375) expressed in COS cells migrates as a 140-150 kDa band.",

keywords = "cDNA sequence, Interferon receptor, Protein crosslinking, α-Interferon",

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T1 - Cloning and characterization of a bovine alpha interferon receptor

AU - Lim, Jin Kyu

AU - Langer, Jerome A.

PY - 1993/6/25

Y1 - 1993/6/25

N2 - A bovine interferon α receptor (BoIFN-αR1) cDNA, homologous to the human cDNA [1], was isolated. Transfection of the BoIFN-αR1 cDNA into monkey COS cells results in a large increase in high-affinity binding sites for human IFN-αA and IFN-αB. Covalent crosslinking of radiolabeled HuIFN-αA and -αB demonstrates that the complex of [32P]HuIFN with the BoIFN-αR1 protein (predicted mass, 61375) expressed in COS cells migrates as a 140-150 kDa band.

AB - A bovine interferon α receptor (BoIFN-αR1) cDNA, homologous to the human cDNA [1], was isolated. Transfection of the BoIFN-αR1 cDNA into monkey COS cells results in a large increase in high-affinity binding sites for human IFN-αA and IFN-αB. Covalent crosslinking of radiolabeled HuIFN-αA and -αB demonstrates that the complex of [32P]HuIFN with the BoIFN-αR1 protein (predicted mass, 61375) expressed in COS cells migrates as a 140-150 kDa band.

KW - cDNA sequence

KW - Interferon receptor

KW - Protein crosslinking

KW - α-Interferon

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U2 - 10.1016/0167-4781(93)90129-2

DO - 10.1016/0167-4781(93)90129-2

M3 - Article

C2 - 8318540

AN - SCOPUS:0027286398

SN - 0167-4781

VL - 1173

SP - 314

EP - 319

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 3

ER -

Cloning and characterization of a bovine alpha interferon receptor

Abstract

Keywords

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