Cloning and characterization of a gene cluster for cyclohexanone oxidation in Rhodococcus sp. TK6

Jun Ho Choi; Tae Kang Kim; Young Mog Kim; Won Chan Kim; Kunbawui Park; In Koo Rhee

Cloning and characterization of a gene cluster for cyclohexanone oxidation in Rhodococcus sp. TK6

Jun Ho Choi
, Tae Kang Kim
, Young Mog Kim
, Won Chan Kim
, Kunbawui Park
, In Koo Rhee

School of Applied Bioscience

Kyungpook National University
Pukyong National University

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A gene cluster for cyclohexanone oxidation was cloned from Rhodococcus sp. TK6, which is capable of growth on cyclohexanone as the sole carbon source. The 9,185-bp DNA sequence analysis revealed seven potential open reading frames (ORFs), designated as ssd-chnR-chnD-chnC-chnB-chnE-partial pcd. The chnBCDE genes encode enzymes for the four-step conversion of cyclohexanone to adipic acid, catalyzed by cyclohexanone monooxygenase (ChnB), ε-caprolactone hydrolase (ChnC), 6-hydroxyhexanoate dehydrogenase (ChnD), and 6-oxohexanoate dehydrogenase (ChnE). Furthermore, the presence of a regulatory element in the downstream region of the chnD gene supports the notion that chnR is a putative regulatory gene. Among them, the activity of ChnB was confirmed and characterized, following their expression and purification in Escherichia coli harboring the modified chnB gene (chnB gene with 6 successive codons for His at the 3′ terminus).

Original language	English
Pages (from-to)	511-518
Number of pages	8
Journal	Journal of Microbiology and Biotechnology
Volume	16
Issue number	4
State	Published - Apr 2006

Keywords

Cyclohexanone monooxygenase
Cyclohexanone oxidation
Rhodococcus sp. TK6

Cite this

@article{f8377040da9841c78af828a1097639e2,

title = "Cloning and characterization of a gene cluster for cyclohexanone oxidation in Rhodococcus sp. TK6",

abstract = "A gene cluster for cyclohexanone oxidation was cloned from Rhodococcus sp. TK6, which is capable of growth on cyclohexanone as the sole carbon source. The 9,185-bp DNA sequence analysis revealed seven potential open reading frames (ORFs), designated as ssd-chnR-chnD-chnC-chnB-chnE-partial pcd. The chnBCDE genes encode enzymes for the four-step conversion of cyclohexanone to adipic acid, catalyzed by cyclohexanone monooxygenase (ChnB), ε-caprolactone hydrolase (ChnC), 6-hydroxyhexanoate dehydrogenase (ChnD), and 6-oxohexanoate dehydrogenase (ChnE). Furthermore, the presence of a regulatory element in the downstream region of the chnD gene supports the notion that chnR is a putative regulatory gene. Among them, the activity of ChnB was confirmed and characterized, following their expression and purification in Escherichia coli harboring the modified chnB gene (chnB gene with 6 successive codons for His at the 3′ terminus).",

keywords = "Cyclohexanone monooxygenase, Cyclohexanone oxidation, Rhodococcus sp. TK6",

author = "Choi, \{Jun Ho\} and Kim, \{Tae Kang\} and Kim, \{Young Mog\} and Kim, \{Won Chan\} and Kunbawui Park and Rhee, \{In Koo\}",

year = "2006",

month = apr,

language = "English",

volume = "16",

pages = "511--518",

journal = "Journal of Microbiology and Biotechnology",

issn = "1017-7825",

publisher = "Korean Society for Microbiolog and Biotechnology",

number = "4",

}

TY - JOUR

T1 - Cloning and characterization of a gene cluster for cyclohexanone oxidation in Rhodococcus sp. TK6

AU - Choi, Jun Ho

AU - Kim, Tae Kang

AU - Kim, Young Mog

AU - Kim, Won Chan

AU - Park, Kunbawui

AU - Rhee, In Koo

PY - 2006/4

Y1 - 2006/4

N2 - A gene cluster for cyclohexanone oxidation was cloned from Rhodococcus sp. TK6, which is capable of growth on cyclohexanone as the sole carbon source. The 9,185-bp DNA sequence analysis revealed seven potential open reading frames (ORFs), designated as ssd-chnR-chnD-chnC-chnB-chnE-partial pcd. The chnBCDE genes encode enzymes for the four-step conversion of cyclohexanone to adipic acid, catalyzed by cyclohexanone monooxygenase (ChnB), ε-caprolactone hydrolase (ChnC), 6-hydroxyhexanoate dehydrogenase (ChnD), and 6-oxohexanoate dehydrogenase (ChnE). Furthermore, the presence of a regulatory element in the downstream region of the chnD gene supports the notion that chnR is a putative regulatory gene. Among them, the activity of ChnB was confirmed and characterized, following their expression and purification in Escherichia coli harboring the modified chnB gene (chnB gene with 6 successive codons for His at the 3′ terminus).

AB - A gene cluster for cyclohexanone oxidation was cloned from Rhodococcus sp. TK6, which is capable of growth on cyclohexanone as the sole carbon source. The 9,185-bp DNA sequence analysis revealed seven potential open reading frames (ORFs), designated as ssd-chnR-chnD-chnC-chnB-chnE-partial pcd. The chnBCDE genes encode enzymes for the four-step conversion of cyclohexanone to adipic acid, catalyzed by cyclohexanone monooxygenase (ChnB), ε-caprolactone hydrolase (ChnC), 6-hydroxyhexanoate dehydrogenase (ChnD), and 6-oxohexanoate dehydrogenase (ChnE). Furthermore, the presence of a regulatory element in the downstream region of the chnD gene supports the notion that chnR is a putative regulatory gene. Among them, the activity of ChnB was confirmed and characterized, following their expression and purification in Escherichia coli harboring the modified chnB gene (chnB gene with 6 successive codons for His at the 3′ terminus).

KW - Cyclohexanone monooxygenase

KW - Cyclohexanone oxidation

KW - Rhodococcus sp. TK6

UR - https://www.scopus.com/pages/publications/33646564983

M3 - Article

AN - SCOPUS:33646564983

SN - 1017-7825

VL - 16

SP - 511

EP - 518

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

IS - 4

ER -

Cloning and characterization of a gene cluster for cyclohexanone oxidation in Rhodococcus sp. TK6

Abstract

Keywords

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