Cloning and characterization of bovine Titin-cap (TCAP) gene

S. L. Yu; H. J. Chung; K. C. Jung; B. C. Sang; D. H. Yoon; S. H. Lee; S. R. Kata; J. E. Womack; J. H. Lee

doi:10.5713/ajas.2004.1344

Cloning and characterization of bovine Titin-cap (TCAP) gene

S. L. Yu, H. J. Chung, K. C. Jung, B. C. Sang, D. H. Yoon, S. H. Lee, S. R. Kata, J. E. Womack, J. H. Lee

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Titin-cap (TCAP), one of the abundant transcripts in skeletal muscles, was investigated in this study in cattle because of its role in regulating the proliferation and differentiation of myoblasts by interacting with the myostatin gene. From the 5′ and 3′ RACE experiments, full-length TCAP coding sequence was identified, comprising 166 amino acids. The amino acid comparison showed high sequence similarities with previously identified human (95.8%) and mouse (95.2%) TCAP genes. The TCAP expression, addressed by northern blot, is limited in muscle tissues as indicated by Valle et al. (1997). The radiation hybrid analysis localized the gene on BTA19, where the comparative human and porcine counterparts are on HSA17 and SSC12. A few muscle-related genetic disorders were mapped on HSA17 and some growth-related QTLs were identified on SSC12. The bovine TCAP gene found in this study opens up new possibilities for the investigation of muscle-related genetic diseases as well as meat yield traits in cattle.

Original language	English
Pages (from-to)	1344-1349
Number of pages	6
Journal	Asian-Australasian Journal of Animal Sciences
Volume	17
Issue number	10
DOIs	https://doi.org/10.5713/ajas.2004.1344
State	Published - Oct 2004

Keywords

Cattle
Northern Analysis
RACE
Radiation Hybrid Mapping
TCAP

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.5713/ajas.2004.1344

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title = "Cloning and characterization of bovine Titin-cap (TCAP) gene",

abstract = "Titin-cap (TCAP), one of the abundant transcripts in skeletal muscles, was investigated in this study in cattle because of its role in regulating the proliferation and differentiation of myoblasts by interacting with the myostatin gene. From the 5′ and 3′ RACE experiments, full-length TCAP coding sequence was identified, comprising 166 amino acids. The amino acid comparison showed high sequence similarities with previously identified human (95.8%) and mouse (95.2%) TCAP genes. The TCAP expression, addressed by northern blot, is limited in muscle tissues as indicated by Valle et al. (1997). The radiation hybrid analysis localized the gene on BTA19, where the comparative human and porcine counterparts are on HSA17 and SSC12. A few muscle-related genetic disorders were mapped on HSA17 and some growth-related QTLs were identified on SSC12. The bovine TCAP gene found in this study opens up new possibilities for the investigation of muscle-related genetic diseases as well as meat yield traits in cattle.",

keywords = "Cattle, Northern Analysis, RACE, Radiation Hybrid Mapping, TCAP",

author = "Yu, {S. L.} and Chung, {H. J.} and Jung, {K. C.} and Sang, {B. C.} and Yoon, {D. H.} and Lee, {S. H.} and Kata, {S. R.} and Womack, {J. E.} and Lee, {J. H.}",

year = "2004",

month = oct,

doi = "10.5713/ajas.2004.1344",

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AU - Yu, S. L.

AU - Chung, H. J.

AU - Jung, K. C.

AU - Sang, B. C.

AU - Yoon, D. H.

AU - Lee, S. H.

AU - Kata, S. R.

AU - Womack, J. E.

AU - Lee, J. H.

PY - 2004/10

Y1 - 2004/10

N2 - Titin-cap (TCAP), one of the abundant transcripts in skeletal muscles, was investigated in this study in cattle because of its role in regulating the proliferation and differentiation of myoblasts by interacting with the myostatin gene. From the 5′ and 3′ RACE experiments, full-length TCAP coding sequence was identified, comprising 166 amino acids. The amino acid comparison showed high sequence similarities with previously identified human (95.8%) and mouse (95.2%) TCAP genes. The TCAP expression, addressed by northern blot, is limited in muscle tissues as indicated by Valle et al. (1997). The radiation hybrid analysis localized the gene on BTA19, where the comparative human and porcine counterparts are on HSA17 and SSC12. A few muscle-related genetic disorders were mapped on HSA17 and some growth-related QTLs were identified on SSC12. The bovine TCAP gene found in this study opens up new possibilities for the investigation of muscle-related genetic diseases as well as meat yield traits in cattle.

AB - Titin-cap (TCAP), one of the abundant transcripts in skeletal muscles, was investigated in this study in cattle because of its role in regulating the proliferation and differentiation of myoblasts by interacting with the myostatin gene. From the 5′ and 3′ RACE experiments, full-length TCAP coding sequence was identified, comprising 166 amino acids. The amino acid comparison showed high sequence similarities with previously identified human (95.8%) and mouse (95.2%) TCAP genes. The TCAP expression, addressed by northern blot, is limited in muscle tissues as indicated by Valle et al. (1997). The radiation hybrid analysis localized the gene on BTA19, where the comparative human and porcine counterparts are on HSA17 and SSC12. A few muscle-related genetic disorders were mapped on HSA17 and some growth-related QTLs were identified on SSC12. The bovine TCAP gene found in this study opens up new possibilities for the investigation of muscle-related genetic diseases as well as meat yield traits in cattle.

KW - Cattle

KW - Northern Analysis

KW - RACE

KW - Radiation Hybrid Mapping

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JO - Asian-Australasian Journal of Animal Sciences

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ER -

Cloning and characterization of bovine Titin-cap (TCAP) gene

Abstract

Keywords

UN SDGs

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