Cloning and sequences of β-actin genes from rhodeus notatus and the silver carp hypophthalmichthys molitrix (Cyprinidae) and the phylogeny of cyprinid fishes inferred from β-actin genes

Ui Wook Hwang; Myung Soo Han; Ii Chan Kim; Yong Sung Lee; Yasunobu Aoki; Jae Seong Lee

doi:10.1080/10425170290034499

Cloning and sequences of β-actin genes from rhodeus notatus and the silver carp hypophthalmichthys molitrix (Cyprinidae) and the phylogeny of cyprinid fishes inferred from β-actin genes

Ui Wook Hwang, Myung Soo Han, Ii Chan Kim, Yong Sung Lee, Yasunobu Aoki, Jae Seong Lee

Department of Biology Education

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Complete β-actin genes from Rhodeus notatus and Hypophthalmichthys molitrix (silver carp) were amplified by polymerase chain reaction and cloned into pCRII vector for further characterization. The β-actin genes of these two fishes were 1718 and 1755 bp in sequence length, respectively, and both of them contained 5 exons and 4 introns. The exon and intron boundaries were identified with the GT/AG rule. The phylogenetic implications of β-actin gene in the family Cyprinidae were shortly discussed.

Original language	English
Pages (from-to)	153-159
Number of pages	7
Journal	Mitochondrial DNA
Volume	13
Issue number	3
DOIs	https://doi.org/10.1080/10425170290034499
State	Published - 2002

Keywords

β-actin
Cyprinid
Intron
Phylogeny

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10.1080/10425170290034499

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title = "Cloning and sequences of β-actin genes from rhodeus notatus and the silver carp hypophthalmichthys molitrix (Cyprinidae) and the phylogeny of cyprinid fishes inferred from β-actin genes",

abstract = "Complete β-actin genes from Rhodeus notatus and Hypophthalmichthys molitrix (silver carp) were amplified by polymerase chain reaction and cloned into pCRII vector for further characterization. The β-actin genes of these two fishes were 1718 and 1755 bp in sequence length, respectively, and both of them contained 5 exons and 4 introns. The exon and intron boundaries were identified with the GT/AG rule. The phylogenetic implications of β-actin gene in the family Cyprinidae were shortly discussed.",

keywords = "β-actin, Cyprinid, Intron, Phylogeny",

author = "Hwang, {Ui Wook} and Han, {Myung Soo} and Kim, {Ii Chan} and Lee, {Yong Sung} and Yasunobu Aoki and Lee, {Jae Seong}",

year = "2002",

doi = "10.1080/10425170290034499",

language = "English",

volume = "13",

pages = "153--159",

journal = "Mitochondrial DNA",

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T1 - Cloning and sequences of β-actin genes from rhodeus notatus and the silver carp hypophthalmichthys molitrix (Cyprinidae) and the phylogeny of cyprinid fishes inferred from β-actin genes

AU - Hwang, Ui Wook

AU - Han, Myung Soo

AU - Kim, Ii Chan

AU - Lee, Yong Sung

AU - Aoki, Yasunobu

AU - Lee, Jae Seong

PY - 2002

Y1 - 2002

N2 - Complete β-actin genes from Rhodeus notatus and Hypophthalmichthys molitrix (silver carp) were amplified by polymerase chain reaction and cloned into pCRII vector for further characterization. The β-actin genes of these two fishes were 1718 and 1755 bp in sequence length, respectively, and both of them contained 5 exons and 4 introns. The exon and intron boundaries were identified with the GT/AG rule. The phylogenetic implications of β-actin gene in the family Cyprinidae were shortly discussed.

AB - Complete β-actin genes from Rhodeus notatus and Hypophthalmichthys molitrix (silver carp) were amplified by polymerase chain reaction and cloned into pCRII vector for further characterization. The β-actin genes of these two fishes were 1718 and 1755 bp in sequence length, respectively, and both of them contained 5 exons and 4 introns. The exon and intron boundaries were identified with the GT/AG rule. The phylogenetic implications of β-actin gene in the family Cyprinidae were shortly discussed.

KW - β-actin

KW - Cyprinid

KW - Intron

KW - Phylogeny

UR - http://www.scopus.com/inward/record.url?scp=0036618032&partnerID=8YFLogxK

U2 - 10.1080/10425170290034499

DO - 10.1080/10425170290034499

M3 - Article

C2 - 12391726

AN - SCOPUS:0036618032

SN - 1940-1736

VL - 13

SP - 153

EP - 159

JO - Mitochondrial DNA

JF - Mitochondrial DNA

IS - 3

ER -

Cloning and sequences of β-actin genes from rhodeus notatus and the silver carp hypophthalmichthys molitrix (Cyprinidae) and the phylogeny of cyprinid fishes inferred from β-actin genes

Abstract

Keywords

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