Cloning and sequences of β-actin genes from rhodeus notatus and the silver carp hypophthalmichthys molitrix (Cyprinidae) and the phylogeny of cyprinid fishes inferred from β-actin genes

Complete β-actin genes from Rhodeus notatus and Hypophthalmichthys molitrix (silver carp) were amplified by polymerase chain reaction and cloned into pCRII vector for further characterization. The β-actin genes of these two fishes were 1718 and 1755 bp in sequence length, respectively, and both of them contained 5 exons and 4 introns. The exon and intron boundaries were identified with the GT/AG rule. The phylogenetic implications of β-actin gene in the family Cyprinidae were shortly discussed.