Cloning of BNIP3h, a member of proapoptotic BNIP3 family genes

Mohammad Farooq; Younghee Kim; Sanguk Im; Eunjung Chung; Sunyoung Hwang; Miyoung Sohn; Moonkyu Kim; Jungchul Kim

doi:10.1038/emm.2001.29

Cloning of BNIP3h, a member of proapoptotic BNIP3 family genes

Mohammad Farooq, Younghee Kim, Sanguk Im, Eunjung Chung, Sunyoung Hwang, Miyoung Sohn, Moonkyu Kim, Jungchul Kim

Department of Medicine

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Apoptosis is regulated by interaction of antiapoptotic Bcl-2 family proteins with various proapoptotic proteins, several of which are also members of the Bcl-2 family. BNIP3 (formerly NIP3) is a proapoptotic mitochondrial protein classified in the Bcl-2 family based on limited sequence homology-3 (BH3) domain and COOH-terminal transmembrane domain. Sequence comparison of BNIP3 has indicated that there are several BNIP3 human homologs of this protein, like BNIP3L, Nix and BNIP3. We have cloned a new member of BNIP3 family from the cDNA library prepared from human dermal papilla cells and designated as BNIP3h. BNIP3h shows substantial homology with other BNIP3 family proteins. BNIP3h induced apoptosis from 24 hours after transfection in MCF7 cell lines and its apoptosis inducing activity is extended until 72 hours after transfection.

Original language	English
Pages (from-to)	169-173
Number of pages	5
Journal	Experimental and Molecular Medicine
Volume	33
Issue number	3
DOIs	https://doi.org/10.1038/emm.2001.29
State	Published - 30 Sep 2001

Keywords

Apoptosis
BNIP3 family proteins
BNIP3h

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10.1038/emm.2001.29

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title = "Cloning of BNIP3h, a member of proapoptotic BNIP3 family genes",

abstract = "Apoptosis is regulated by interaction of antiapoptotic Bcl-2 family proteins with various proapoptotic proteins, several of which are also members of the Bcl-2 family. BNIP3 (formerly NIP3) is a proapoptotic mitochondrial protein classified in the Bcl-2 family based on limited sequence homology-3 (BH3) domain and COOH-terminal transmembrane domain. Sequence comparison of BNIP3 has indicated that there are several BNIP3 human homologs of this protein, like BNIP3L, Nix and BNIP3. We have cloned a new member of BNIP3 family from the cDNA library prepared from human dermal papilla cells and designated as BNIP3h. BNIP3h shows substantial homology with other BNIP3 family proteins. BNIP3h induced apoptosis from 24 hours after transfection in MCF7 cell lines and its apoptosis inducing activity is extended until 72 hours after transfection.",

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AU - Farooq, Mohammad

AU - Kim, Younghee

AU - Im, Sanguk

AU - Chung, Eunjung

AU - Hwang, Sunyoung

AU - Sohn, Miyoung

AU - Kim, Moonkyu

AU - Kim, Jungchul

PY - 2001/9/30

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N2 - Apoptosis is regulated by interaction of antiapoptotic Bcl-2 family proteins with various proapoptotic proteins, several of which are also members of the Bcl-2 family. BNIP3 (formerly NIP3) is a proapoptotic mitochondrial protein classified in the Bcl-2 family based on limited sequence homology-3 (BH3) domain and COOH-terminal transmembrane domain. Sequence comparison of BNIP3 has indicated that there are several BNIP3 human homologs of this protein, like BNIP3L, Nix and BNIP3. We have cloned a new member of BNIP3 family from the cDNA library prepared from human dermal papilla cells and designated as BNIP3h. BNIP3h shows substantial homology with other BNIP3 family proteins. BNIP3h induced apoptosis from 24 hours after transfection in MCF7 cell lines and its apoptosis inducing activity is extended until 72 hours after transfection.

AB - Apoptosis is regulated by interaction of antiapoptotic Bcl-2 family proteins with various proapoptotic proteins, several of which are also members of the Bcl-2 family. BNIP3 (formerly NIP3) is a proapoptotic mitochondrial protein classified in the Bcl-2 family based on limited sequence homology-3 (BH3) domain and COOH-terminal transmembrane domain. Sequence comparison of BNIP3 has indicated that there are several BNIP3 human homologs of this protein, like BNIP3L, Nix and BNIP3. We have cloned a new member of BNIP3 family from the cDNA library prepared from human dermal papilla cells and designated as BNIP3h. BNIP3h shows substantial homology with other BNIP3 family proteins. BNIP3h induced apoptosis from 24 hours after transfection in MCF7 cell lines and its apoptosis inducing activity is extended until 72 hours after transfection.

KW - Apoptosis

KW - BNIP3 family proteins

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Cloning of BNIP3h, a member of proapoptotic BNIP3 family genes

Abstract

Keywords

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