Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression

J. I. Chae; S. K. Ju; M. K. Lee; J. H. Park; J. H. Shim; K. K. Lee; D. S. Lee

doi:10.1134/S0026893308040122

Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression

J. I. Chae, S. K. Ju, M. K. Lee, J. H. Park, J. H. Shim, K. K. Lee, D. S. Lee

Research output: Contribution to journal › Article › peer-review

Abstract

Thymus-and activation-regulated chemokine (TARC) is one that selectively controls the migration of type 2-helper T lymphocytes into inflammatory lesions. TARC is a CC chemokine and plays an essential role in recruiting CC chemokine receptor 4-positive Th2 cells to allergic lesions. We cloned TARC cDNA from rat thymus using RT-PCR. The rat TARC clone contained a full-length open reading frame encoding 93 amino acids that showed 83 and 66% homology with mouse and human homologs, respectively. The expression of TARC mRNA was mainly in the lymphoid organs, for example, the thymus, spleen, and lymph node. The recombinant TARC was expressed in Escherichia coli and purified in an active form. In addition, the purified rat TARC with S-tagged specifically binds to human CCR4 in CD4/CCR4-transfected HOS cells by cell-binding assay using flow cytometry. The TARC cDNA clones obtained in this study will be valuable for future studies on allergic diseases in rats.

Original language	English
Pages (from-to)	567-571
Number of pages	5
Journal	Molecular Biology
Volume	42
Issue number	4
DOIs	https://doi.org/10.1134/S0026893308040122
State	Published - Aug 2008

Keywords

CCR4
Chemokine
Immunological regulation
Molecular immunoligy
TARC

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1134/S0026893308040122

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title = "Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression",

abstract = "Thymus-and activation-regulated chemokine (TARC) is one that selectively controls the migration of type 2-helper T lymphocytes into inflammatory lesions. TARC is a CC chemokine and plays an essential role in recruiting CC chemokine receptor 4-positive Th2 cells to allergic lesions. We cloned TARC cDNA from rat thymus using RT-PCR. The rat TARC clone contained a full-length open reading frame encoding 93 amino acids that showed 83 and 66% homology with mouse and human homologs, respectively. The expression of TARC mRNA was mainly in the lymphoid organs, for example, the thymus, spleen, and lymph node. The recombinant TARC was expressed in Escherichia coli and purified in an active form. In addition, the purified rat TARC with S-tagged specifically binds to human CCR4 in CD4/CCR4-transfected HOS cells by cell-binding assay using flow cytometry. The TARC cDNA clones obtained in this study will be valuable for future studies on allergic diseases in rats.",

keywords = "CCR4, Chemokine, Immunological regulation, Molecular immunoligy, TARC",

author = "Chae, {J. I.} and Ju, {S. K.} and Lee, {M. K.} and Park, {J. H.} and Shim, {J. H.} and Lee, {K. K.} and Lee, {D. S.}",

year = "2008",

month = aug,

doi = "10.1134/S0026893308040122",

language = "English",

volume = "42",

pages = "567--571",

journal = "Molecular Biology",

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T1 - Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression

AU - Chae, J. I.

AU - Ju, S. K.

AU - Lee, M. K.

AU - Park, J. H.

AU - Shim, J. H.

AU - Lee, K. K.

AU - Lee, D. S.

PY - 2008/8

Y1 - 2008/8

N2 - Thymus-and activation-regulated chemokine (TARC) is one that selectively controls the migration of type 2-helper T lymphocytes into inflammatory lesions. TARC is a CC chemokine and plays an essential role in recruiting CC chemokine receptor 4-positive Th2 cells to allergic lesions. We cloned TARC cDNA from rat thymus using RT-PCR. The rat TARC clone contained a full-length open reading frame encoding 93 amino acids that showed 83 and 66% homology with mouse and human homologs, respectively. The expression of TARC mRNA was mainly in the lymphoid organs, for example, the thymus, spleen, and lymph node. The recombinant TARC was expressed in Escherichia coli and purified in an active form. In addition, the purified rat TARC with S-tagged specifically binds to human CCR4 in CD4/CCR4-transfected HOS cells by cell-binding assay using flow cytometry. The TARC cDNA clones obtained in this study will be valuable for future studies on allergic diseases in rats.

AB - Thymus-and activation-regulated chemokine (TARC) is one that selectively controls the migration of type 2-helper T lymphocytes into inflammatory lesions. TARC is a CC chemokine and plays an essential role in recruiting CC chemokine receptor 4-positive Th2 cells to allergic lesions. We cloned TARC cDNA from rat thymus using RT-PCR. The rat TARC clone contained a full-length open reading frame encoding 93 amino acids that showed 83 and 66% homology with mouse and human homologs, respectively. The expression of TARC mRNA was mainly in the lymphoid organs, for example, the thymus, spleen, and lymph node. The recombinant TARC was expressed in Escherichia coli and purified in an active form. In addition, the purified rat TARC with S-tagged specifically binds to human CCR4 in CD4/CCR4-transfected HOS cells by cell-binding assay using flow cytometry. The TARC cDNA clones obtained in this study will be valuable for future studies on allergic diseases in rats.

KW - CCR4

KW - Chemokine

KW - Immunological regulation

KW - Molecular immunoligy

KW - TARC

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U2 - 10.1134/S0026893308040122

DO - 10.1134/S0026893308040122

M3 - Article

AN - SCOPUS:49549115551

SN - 0026-8933

VL - 42

SP - 567

EP - 571

JO - Molecular Biology

JF - Molecular Biology

IS - 4

ER -

Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression

Abstract

Keywords

UN SDGs

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