Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression

J. I. Chae; S. K. Ju; M. K. Lee; J. H. Park; J. H. Shim; K. K. Lee; D. S. Lee

Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression

J. I. Chae, S. K. Ju, M. K. Lee, J. H. Park, J. H. Shim, K. K. Lee, D. S. Lee

Research output: Contribution to journal › Article › peer-review

Abstract

Thymus and activation-regulated chemokine (TARC) is one that selectively controls the migration of type 2-helper T lymphocytes into inflammatory lesions. TARC is a CC chemokine, and plays an essential role in recruiting CC chemokine receptor 4-positive Th2 cells to allergic lesions. We cloned TARC cDNA from rat thymus using RT-PCR. The rat TARC clone contained a full-length open reading frame encoding 93 amino acids that showed 83% and 66% homology with mouse and human homologs, respectively. The expression of TARC mRNA was mainly in the lymphoid organs, for example, the thymus, spleen, and lymph node. The recombinant TARC was expressed in Escherichia coli and purified in an active form. In addition, the purified rat TARC with S-tagged specifically binds to human CCR4 in CD4.CCR4-transfected HOS cells by Cell-binding assay using flow-cytometry. The TARC cDNA clones obtained in this study will be valuable for future studies on allergic diseases in rats.

Original language	English
Pages (from-to)	639-644
Number of pages	6
Journal	Molekulyarnaya Biologiya
Volume	42
Issue number	4
State	Published - Jul 2008

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title = "Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression",

abstract = "Thymus and activation-regulated chemokine (TARC) is one that selectively controls the migration of type 2-helper T lymphocytes into inflammatory lesions. TARC is a CC chemokine, and plays an essential role in recruiting CC chemokine receptor 4-positive Th2 cells to allergic lesions. We cloned TARC cDNA from rat thymus using RT-PCR. The rat TARC clone contained a full-length open reading frame encoding 93 amino acids that showed 83% and 66% homology with mouse and human homologs, respectively. The expression of TARC mRNA was mainly in the lymphoid organs, for example, the thymus, spleen, and lymph node. The recombinant TARC was expressed in Escherichia coli and purified in an active form. In addition, the purified rat TARC with S-tagged specifically binds to human CCR4 in CD4.CCR4-transfected HOS cells by Cell-binding assay using flow-cytometry. The TARC cDNA clones obtained in this study will be valuable for future studies on allergic diseases in rats.",

author = "Chae, {J. I.} and Ju, {S. K.} and Lee, {M. K.} and Park, {J. H.} and Shim, {J. H.} and Lee, {K. K.} and Lee, {D. S.}",

year = "2008",

month = jul,

language = "English",

volume = "42",

pages = "639--644",

journal = "Molekulyarnaya Biologiya",

issn = "0026-8984",

publisher = "Russian Academy of Sciences",

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TY - JOUR

T1 - Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression

AU - Chae, J. I.

AU - Ju, S. K.

AU - Lee, M. K.

AU - Park, J. H.

AU - Shim, J. H.

AU - Lee, K. K.

AU - Lee, D. S.

PY - 2008/7

Y1 - 2008/7

N2 - Thymus and activation-regulated chemokine (TARC) is one that selectively controls the migration of type 2-helper T lymphocytes into inflammatory lesions. TARC is a CC chemokine, and plays an essential role in recruiting CC chemokine receptor 4-positive Th2 cells to allergic lesions. We cloned TARC cDNA from rat thymus using RT-PCR. The rat TARC clone contained a full-length open reading frame encoding 93 amino acids that showed 83% and 66% homology with mouse and human homologs, respectively. The expression of TARC mRNA was mainly in the lymphoid organs, for example, the thymus, spleen, and lymph node. The recombinant TARC was expressed in Escherichia coli and purified in an active form. In addition, the purified rat TARC with S-tagged specifically binds to human CCR4 in CD4.CCR4-transfected HOS cells by Cell-binding assay using flow-cytometry. The TARC cDNA clones obtained in this study will be valuable for future studies on allergic diseases in rats.

AB - Thymus and activation-regulated chemokine (TARC) is one that selectively controls the migration of type 2-helper T lymphocytes into inflammatory lesions. TARC is a CC chemokine, and plays an essential role in recruiting CC chemokine receptor 4-positive Th2 cells to allergic lesions. We cloned TARC cDNA from rat thymus using RT-PCR. The rat TARC clone contained a full-length open reading frame encoding 93 amino acids that showed 83% and 66% homology with mouse and human homologs, respectively. The expression of TARC mRNA was mainly in the lymphoid organs, for example, the thymus, spleen, and lymph node. The recombinant TARC was expressed in Escherichia coli and purified in an active form. In addition, the purified rat TARC with S-tagged specifically binds to human CCR4 in CD4.CCR4-transfected HOS cells by Cell-binding assay using flow-cytometry. The TARC cDNA clones obtained in this study will be valuable for future studies on allergic diseases in rats.

UR - http://www.scopus.com/inward/record.url?scp=55249089214&partnerID=8YFLogxK

M3 - Article

C2 - 18856064

AN - SCOPUS:55249089214

SN - 0026-8984

VL - 42

SP - 639

EP - 644

JO - Molekulyarnaya Biologiya

JF - Molekulyarnaya Biologiya

IS - 4

ER -

Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression

Abstract

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