Combined radionuclide-chemotherapy and in vivo imaging of hepatocellular carcinoma cells after transfection of a triple-gene construct, NIS, HSV1-sr39tk, and EGFP

You La Lee; Yong Jin Lee; Sohn Joo Ahn; Tae Hyun Choi; Byung Seok Moon; Gi Jeong Cheon; Sang Woo Lee; Byeong Cheol Ahn; Jeoung Hee Ha; Jaetae Lee

doi:10.1016/j.canlet.2009.09.004

Combined radionuclide-chemotherapy and in vivo imaging of hepatocellular carcinoma cells after transfection of a triple-gene construct, NIS, HSV1-sr39tk, and EGFP

You La Lee, Yong Jin Lee, Sohn Joo Ahn, Tae Hyun Choi, Byung Seok Moon, Gi Jeong Cheon, Sang Woo Lee, Byeong Cheol Ahn, Jeoung Hee Ha, Jaetae Lee

Department of Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The sodium iodine symporter (NIS) or mutant Herpes-simplex virus type1 sr39 thymidine kinase (HSV1-sr39tk) gene is used for in vivo imaging and cancer therapy. Transfection of both NIS and HSV1-sr39tk genes to hepatocellular carcinoma cells (Huh-7/NTG) could enhance intracellular accumulation of therapeutic radionuclides and guanosine nucleoside analogue prodrugs to produce better outcomes than single gene therapy. Non-invasive imaging with I-124, F-18 FHBG and combination therapy with I-131 and GCV were performed in hepatocellular carcinoma cells transfected with NIS, HSV1-sr39tk and GFP. Our results show that: (1) all three genes are stably expressed in Huh-7/NTG cells, (2) I-125 and H3-PCV uptake were markedly increased in the Huh-7/NTG cells in vitro, (3) cellular survival and tumor growth of Huh-7/NTG was inhibited by I-131 or GCV both in vitro and in vivo, and was much prominent with combination therapy, (4) in vivo imaging with I-124 and F-18 FHBG revealed increased uptake in the Huh-7/NTG tumor. Our results demonstrated the potential of combination gene therapy using NIS and HSV1-sr39tk followed by radioiodine treatment and chemotherapy in human hepatocellular carcinoma cells.

Original language	English
Pages (from-to)	129-138
Number of pages	10
Journal	Cancer Letters
Volume	290
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2009.09.004
State	Published - 1 Apr 2010

Keywords

EGFP
Human sodium iodide symporter
I-124
Mutant herpes-simplex virus type1 sr39 thymidine kinase
Triple-reporter gene

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2009.09.004

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Lee, Y. L., Lee, Y. J., Ahn, S. J., Choi, T. H., Moon, B. S., Cheon, G. J., Lee, S. W., Ahn, B. C., Ha, J. H., & Lee, J. (2010). Combined radionuclide-chemotherapy and in vivo imaging of hepatocellular carcinoma cells after transfection of a triple-gene construct, NIS, HSV1-sr39tk, and EGFP. Cancer Letters, 290(1), 129-138. https://doi.org/10.1016/j.canlet.2009.09.004

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title = "Combined radionuclide-chemotherapy and in vivo imaging of hepatocellular carcinoma cells after transfection of a triple-gene construct, NIS, HSV1-sr39tk, and EGFP",

abstract = "The sodium iodine symporter (NIS) or mutant Herpes-simplex virus type1 sr39 thymidine kinase (HSV1-sr39tk) gene is used for in vivo imaging and cancer therapy. Transfection of both NIS and HSV1-sr39tk genes to hepatocellular carcinoma cells (Huh-7/NTG) could enhance intracellular accumulation of therapeutic radionuclides and guanosine nucleoside analogue prodrugs to produce better outcomes than single gene therapy. Non-invasive imaging with I-124, F-18 FHBG and combination therapy with I-131 and GCV were performed in hepatocellular carcinoma cells transfected with NIS, HSV1-sr39tk and GFP. Our results show that: (1) all three genes are stably expressed in Huh-7/NTG cells, (2) I-125 and H3-PCV uptake were markedly increased in the Huh-7/NTG cells in vitro, (3) cellular survival and tumor growth of Huh-7/NTG was inhibited by I-131 or GCV both in vitro and in vivo, and was much prominent with combination therapy, (4) in vivo imaging with I-124 and F-18 FHBG revealed increased uptake in the Huh-7/NTG tumor. Our results demonstrated the potential of combination gene therapy using NIS and HSV1-sr39tk followed by radioiodine treatment and chemotherapy in human hepatocellular carcinoma cells.",

keywords = "EGFP, Human sodium iodide symporter, I-124, Mutant herpes-simplex virus type1 sr39 thymidine kinase, Triple-reporter gene",

author = "Lee, {You La} and Lee, {Yong Jin} and Ahn, {Sohn Joo} and Choi, {Tae Hyun} and Moon, {Byung Seok} and Cheon, {Gi Jeong} and Lee, {Sang Woo} and Ahn, {Byeong Cheol} and Ha, {Jeoung Hee} and Jaetae Lee",

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doi = "10.1016/j.canlet.2009.09.004",

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AU - Lee, You La

AU - Lee, Yong Jin

AU - Ahn, Sohn Joo

AU - Choi, Tae Hyun

AU - Moon, Byung Seok

AU - Cheon, Gi Jeong

AU - Lee, Sang Woo

AU - Ahn, Byeong Cheol

AU - Ha, Jeoung Hee

AU - Lee, Jaetae

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AB - The sodium iodine symporter (NIS) or mutant Herpes-simplex virus type1 sr39 thymidine kinase (HSV1-sr39tk) gene is used for in vivo imaging and cancer therapy. Transfection of both NIS and HSV1-sr39tk genes to hepatocellular carcinoma cells (Huh-7/NTG) could enhance intracellular accumulation of therapeutic radionuclides and guanosine nucleoside analogue prodrugs to produce better outcomes than single gene therapy. Non-invasive imaging with I-124, F-18 FHBG and combination therapy with I-131 and GCV were performed in hepatocellular carcinoma cells transfected with NIS, HSV1-sr39tk and GFP. Our results show that: (1) all three genes are stably expressed in Huh-7/NTG cells, (2) I-125 and H3-PCV uptake were markedly increased in the Huh-7/NTG cells in vitro, (3) cellular survival and tumor growth of Huh-7/NTG was inhibited by I-131 or GCV both in vitro and in vivo, and was much prominent with combination therapy, (4) in vivo imaging with I-124 and F-18 FHBG revealed increased uptake in the Huh-7/NTG tumor. Our results demonstrated the potential of combination gene therapy using NIS and HSV1-sr39tk followed by radioiodine treatment and chemotherapy in human hepatocellular carcinoma cells.

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KW - Human sodium iodide symporter

KW - I-124

KW - Mutant herpes-simplex virus type1 sr39 thymidine kinase

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Combined radionuclide-chemotherapy and in vivo imaging of hepatocellular carcinoma cells after transfection of a triple-gene construct, NIS, HSV1-sr39tk, and EGFP

Abstract

Keywords

UN SDGs

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