Comparative sequence analysis and adipose-specific expression of G0S2 and ATGL in sheep

Jinsoo Ahn, Huiguang Wu, Yeunsu Suh, Ana C. Carranza, Alejandro E. Relling, Sangsu Shin, Sang Suk Lee, Kichoon Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Lipolysis is a catabolic reaction that is mediated by adipose triglyceride lipase (ATGL) and its inhibitor such as G0/G1 switch gene 2 (G0S2) protein during the first step of hydrolysis. The hydrophobic domain of G0S2 protein interacts and binds to the patatin-like domain in ATGL. Although conservation of the hydrophobic domain has been reported across species, sheep G0S2 amino acids and its hydrophobic domain have not been sequenced and studied. In this study, the hydrophobic region of G0S2 which plays an important role in inhibition of ATGL-mediated lipolysis was highly conserved as shown in other species. Phylogenetic analysis revealed that ruminant G0S2 proteins showed greater homologies within the ruminant species compared to other mammalian species. Moreover, adipose-specific expression of G0S2 for sheep was observed. Together with an abundant expression of ATGL protein in adipose tissue of sheep, G0S2 protein may play a critical role in inhibiting ATGL-mediated triacylglycerol (TAG) hydrolysis in the adipose tissue.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalSmall Ruminant Research
Volume153
DOIs
StatePublished - 1 Aug 2017

Keywords

  • Adipose triglyceride lipase (ATGL)
  • Adipose-specific expression
  • G0/G1 switch gene 2 (G0S2)
  • Hydrophobic domain
  • Lipolysis
  • Sheep

Fingerprint

Dive into the research topics of 'Comparative sequence analysis and adipose-specific expression of G0S2 and ATGL in sheep'. Together they form a unique fingerprint.

Cite this