Comparison of two PCR assays for trichomonas vaginalis

Chang Suk Noh; Sang Su Kim; Sung Yul Park; Hong Sang Moon; Yeonchul Hong; Jae Sook Ryu

doi:10.3347/kjp.2019.57.1.27

Comparison of two PCR assays for trichomonas vaginalis

Chang Suk Noh, Sang Su Kim, Sung Yul Park, Hong Sang Moon, Yeonchul Hong, Jae Sook Ryu

Department of Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

Original language	English
Pages (from-to)	27-31
Number of pages	5
Journal	Korean Journal of Parasitology
Volume	57
Issue number	1
DOIs	https://doi.org/10.3347/kjp.2019.57.1.27
State	Published - Feb 2019

Keywords

DNA isolation
PCR
Trichomonas vaginalis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3347/kjp.2019.57.1.27

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title = "Comparison of two PCR assays for trichomonas vaginalis",

abstract = "PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit{\textregistered}, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.",

keywords = "DNA isolation, PCR, Trichomonas vaginalis",

author = "Noh, \{Chang Suk\} and Kim, \{Sang Su\} and Park, \{Sung Yul\} and Moon, \{Hong Sang\} and Yeonchul Hong and Ryu, \{Jae Sook\}",

note = "Publisher Copyright: {\textcopyright} 2019, Korean Society for Parasitology and Tropical Medicine.",

year = "2019",

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doi = "10.3347/kjp.2019.57.1.27",

language = "English",

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T1 - Comparison of two PCR assays for trichomonas vaginalis

AU - Noh, Chang Suk

AU - Kim, Sang Su

AU - Park, Sung Yul

AU - Moon, Hong Sang

AU - Hong, Yeonchul

AU - Ryu, Jae Sook

PY - 2019/2

Y1 - 2019/2

N2 - PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

AB - PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

KW - DNA isolation

KW - PCR

KW - Trichomonas vaginalis

UR - https://www.scopus.com/pages/publications/85062602270

U2 - 10.3347/kjp.2019.57.1.27

DO - 10.3347/kjp.2019.57.1.27

M3 - Article

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AN - SCOPUS:85062602270

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VL - 57

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EP - 31

JO - Korean Journal of Parasitology

JF - Korean Journal of Parasitology

IS - 1

ER -

Comparison of two PCR assays for trichomonas vaginalis

Abstract

Keywords

UN SDGs

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